MicroRNA expression analysis by LNA enhanced microarrays
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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MicroRNA expression analysis by LNA enhanced microarrays. / Søkilde, Rolf; Kaczkowski, Bogumil; Barken, Kim Bundvig; Mouritzen, Peter; Møller, Søren; Litman, Thomas.
MicroRNA Profiling in Cancer: A Bioinformatics Perspective. Pan Stanford Publishing Pte. Ltd., 2009. s. 23-46.Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - MicroRNA expression analysis by LNA enhanced microarrays
AU - Søkilde, Rolf
AU - Kaczkowski, Bogumil
AU - Barken, Kim Bundvig
AU - Mouritzen, Peter
AU - Møller, Søren
AU - Litman, Thomas
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Array expression profiling of miRNAs has become a widely used technique and thereby gained a broader user spectrum from novice to highly experienced array specialists. In this chapter we discuss the challenges in designing microarrays for miRNA detection and show the advantages of LNA™-enhanced capture probes. We cover important aspects of array normalization and show its practical application in a case study with miRNA data from the NCI-60 cell line panel. We also demonstrate that commonly used methods for normalization of mRNA expression arrays can be applied for miRNA array data. These methods are implemented in both open source and commercial software.
AB - Array expression profiling of miRNAs has become a widely used technique and thereby gained a broader user spectrum from novice to highly experienced array specialists. In this chapter we discuss the challenges in designing microarrays for miRNA detection and show the advantages of LNA™-enhanced capture probes. We cover important aspects of array normalization and show its practical application in a case study with miRNA data from the NCI-60 cell line panel. We also demonstrate that commonly used methods for normalization of mRNA expression arrays can be applied for miRNA array data. These methods are implemented in both open source and commercial software.
UR - http://www.scopus.com/inward/record.url?scp=79955747768&partnerID=8YFLogxK
U2 - 10.4032/9789814267540
DO - 10.4032/9789814267540
M3 - Book chapter
AN - SCOPUS:79955747768
SN - 9789814267014
SP - 23
EP - 46
BT - MicroRNA Profiling in Cancer
PB - Pan Stanford Publishing Pte. Ltd.
ER -
ID: 200582084