Mechanism of action of compound-13: An α1-selective small molecule activator of AMPK
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Mechanism of action of compound-13 : An α1-selective small molecule activator of AMPK. / Hunter, Roger W.; Foretz, Marc; Bultot, Laurent; Fullerton, Morgan D.; Deak, Maria; Ross, Fiona A.; Hawley, Simon A.; Shpiro, Natalia; Viollet, Benoit; Barron, Denis; Kemp, Bruce E.; Steinberg, Gregory R.; Hardie, D. Grahame; Sakamoto, Kei.
I: Chemistry and Biology, Bind 21, Nr. 7, 17.07.2014, s. 866-879.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Mechanism of action of compound-13
T2 - An α1-selective small molecule activator of AMPK
AU - Hunter, Roger W.
AU - Foretz, Marc
AU - Bultot, Laurent
AU - Fullerton, Morgan D.
AU - Deak, Maria
AU - Ross, Fiona A.
AU - Hawley, Simon A.
AU - Shpiro, Natalia
AU - Viollet, Benoit
AU - Barron, Denis
AU - Kemp, Bruce E.
AU - Steinberg, Gregory R.
AU - Hardie, D. Grahame
AU - Sakamoto, Kei
PY - 2014/7/17
Y1 - 2014/7/17
N2 - Summary AMPK is a sensor of cellular energy status and a promising target for drugs aimed at metabolic disorders. We have studied the selectivity and mechanism of a recently described activator, C2, and its cell-permeable prodrug, C13. C2 was a potent allosteric activator of α1-complexes that, like AMP, also protected against Thr172 dephosphorylation. Compared with AMP, C2 caused only partial allosteric activation of α2-complexes and failed to protect them against dephosphorylation. We show that both effects could be fully restored by exchanging part of the linker between the autoinhibitory and C-terminal domains in α2, containing the equivalent region from α1 thought to interact with AMP bound in site 3 of the γ subunit. Consistent with our results in cell-free assays, C13 potently inhibited lipid synthesis in hepatocytes from wild-type and was largely ineffective in AMPK-knockout hepatocytes; its effects were more severely affected by knockout of α1 than of α2, β1, or β2.
AB - Summary AMPK is a sensor of cellular energy status and a promising target for drugs aimed at metabolic disorders. We have studied the selectivity and mechanism of a recently described activator, C2, and its cell-permeable prodrug, C13. C2 was a potent allosteric activator of α1-complexes that, like AMP, also protected against Thr172 dephosphorylation. Compared with AMP, C2 caused only partial allosteric activation of α2-complexes and failed to protect them against dephosphorylation. We show that both effects could be fully restored by exchanging part of the linker between the autoinhibitory and C-terminal domains in α2, containing the equivalent region from α1 thought to interact with AMP bound in site 3 of the γ subunit. Consistent with our results in cell-free assays, C13 potently inhibited lipid synthesis in hepatocytes from wild-type and was largely ineffective in AMPK-knockout hepatocytes; its effects were more severely affected by knockout of α1 than of α2, β1, or β2.
UR - http://www.scopus.com/inward/record.url?scp=84904556335&partnerID=8YFLogxK
U2 - 10.1016/j.chembiol.2014.05.014
DO - 10.1016/j.chembiol.2014.05.014
M3 - Journal article
C2 - 25036776
AN - SCOPUS:84904556335
VL - 21
SP - 866
EP - 879
JO - Chemistry and Biology
JF - Chemistry and Biology
SN - 2451-9448
IS - 7
ER -
ID: 239213236