Measurement of capillary permeability in canine heart determined by the tissue injection, residue detection method
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Measurement of capillary permeability in canine heart determined by the tissue injection, residue detection method. / Svendsen, Jesper Hastrup; Paaske, W P; Haunsø, S; Sejrsen, P.
I: Acta Physiologica Scandinavica. Supplementum, Bind 603, 01.01.1991, s. 133-40.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Measurement of capillary permeability in canine heart determined by the tissue injection, residue detection method
AU - Svendsen, Jesper Hastrup
AU - Paaske, W P
AU - Haunsø, S
AU - Sejrsen, P
PY - 1991/1/1
Y1 - 1991/1/1
N2 - In previous studies the tissue injection, residue detection method failed to provide results of diffusional capillary permeability comparable to those of other methods. For this reason we reconsidered the kinetic theory and found that it is necessary to take into account the apparent (restricted) diffusion coefficient of the indicator within the tissue, D', in order to determine the permeability-surface area product, PdS = Vev.D.D'-1.tev-1 = Vev'.klo where D is the diffusion coefficient in free aqueous solution, Vev is the physical interstitial water volume of distribution, Vev' is the virtual (apparent) interstitial volume of distribution, tev is the mean transit time of the indicator, and klo is the recorded fractional initial washout rate constant. In experiments on open chest dog hearts we examined capillary permeability for 51Cr-EDTA and 99mTc-DTPA with the tissue injection, residue detection method and the single injection, residue detection method. Blood flow was measured independently with local 133Xenon washout. D and D' were measured by a true transient diffusion method. We found that the tissue injection, residue detection method gave results for capillary extraction and PdS-product similar to those obtained with a number of other methods based on indicator diffusion, so the tissue injection, residue detection method in its new kinetically correct formulation could prove useful in clinical studies of capillary permeability since it is applicable to determination of relative changes of permeability during interventional procedures.
AB - In previous studies the tissue injection, residue detection method failed to provide results of diffusional capillary permeability comparable to those of other methods. For this reason we reconsidered the kinetic theory and found that it is necessary to take into account the apparent (restricted) diffusion coefficient of the indicator within the tissue, D', in order to determine the permeability-surface area product, PdS = Vev.D.D'-1.tev-1 = Vev'.klo where D is the diffusion coefficient in free aqueous solution, Vev is the physical interstitial water volume of distribution, Vev' is the virtual (apparent) interstitial volume of distribution, tev is the mean transit time of the indicator, and klo is the recorded fractional initial washout rate constant. In experiments on open chest dog hearts we examined capillary permeability for 51Cr-EDTA and 99mTc-DTPA with the tissue injection, residue detection method and the single injection, residue detection method. Blood flow was measured independently with local 133Xenon washout. D and D' were measured by a true transient diffusion method. We found that the tissue injection, residue detection method gave results for capillary extraction and PdS-product similar to those obtained with a number of other methods based on indicator diffusion, so the tissue injection, residue detection method in its new kinetically correct formulation could prove useful in clinical studies of capillary permeability since it is applicable to determination of relative changes of permeability during interventional procedures.
KW - Animals
KW - Capillary Permeability
KW - Diffusion
KW - Dogs
KW - Edetic Acid
KW - Methods
KW - Myocardium
KW - Technetium Tc 99m Pentetate
M3 - Journal article
C2 - 1789124
VL - 603
SP - 133
EP - 140
JO - Acta Physiologica Scandinavica
JF - Acta Physiologica Scandinavica
SN - 0001-6772
ER -
ID: 32476991