m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site

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Standard

m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site. / Parker, Matthew T.; Soanes, Beth K.; Kusakina, Jelena; Larrieu, Antoine; Knop, Katarzyna; Joy, Nisha; Breidenbach, Friedrich; Sherwood, Anna; Barton, Geoffrey J.; Fica, Sebastian M.; Davies, Brendan H.; Simpson, Gordon G.

I: eLife, Bind 11, 78808, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Parker, MT, Soanes, BK, Kusakina, J, Larrieu, A, Knop, K, Joy, N, Breidenbach, F, Sherwood, A, Barton, GJ, Fica, SM, Davies, BH & Simpson, GG 2022, 'm6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site', eLife, bind 11, 78808. https://doi.org/10.7554/eLife.78808

APA

Parker, M. T., Soanes, B. K., Kusakina, J., Larrieu, A., Knop, K., Joy, N., Breidenbach, F., Sherwood, A., Barton, G. J., Fica, S. M., Davies, B. H., & Simpson, G. G. (2022). m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site. eLife, 11, [78808]. https://doi.org/10.7554/eLife.78808

Vancouver

Parker MT, Soanes BK, Kusakina J, Larrieu A, Knop K, Joy N o.a. m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site. eLife. 2022;11. 78808. https://doi.org/10.7554/eLife.78808

Author

Parker, Matthew T. ; Soanes, Beth K. ; Kusakina, Jelena ; Larrieu, Antoine ; Knop, Katarzyna ; Joy, Nisha ; Breidenbach, Friedrich ; Sherwood, Anna ; Barton, Geoffrey J. ; Fica, Sebastian M. ; Davies, Brendan H. ; Simpson, Gordon G. / m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site. I: eLife. 2022 ; Bind 11.

Bibtex

@article{5f871eff74864c9799a54f63a4e537c8,
title = "m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site",
abstract = "Alternative splicing of messenger RNAs is associated with the evolution of developmentally complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA 5' splice site into the spliceosome active site depends upon pairing with the conserved ACAGA sequence of U6 snRNA. In some species, including humans, the central adenosine of the ACAGA box is modified by N-6 methylation, but the role of this m(6)A modification is poorly understood. Here, we show that m(6)A modified U6 snRNA determines the accuracy and efficiency of splicing. We reveal that the conserved methyltransferase, FIONA1, is required for Arabidopsis U6 snRNA m(6)A modification. Arabidopsis fio1 mutants show disrupted patterns of splicing that can be explained by the sequence composition of 5' splice sites and cooperative roles for U5 and U6 snRNA in splice site selection. U6 snRNA m(6)A influences 3' splice site usage. We generalise these findings to reveal two major classes of 5' splice site in diverse eukaryotes, which display anti-correlated interaction potential with U5 snRNA loop 1 and the U6 snRNA ACAGA box. We conclude that U6 snRNA m(6)A modification contributes to the selection of degenerate 5' splice sites crucial to alternative splicing.",
keywords = "splicing, epitranscriptome, m6A, flowering time, ambient temperature, A, thaliana, C, elegans, D, melanogaster, Human, Zebrafish, SMALL NUCLEAR RNAS, CRYO-EM STRUCTURE, N-6-ADENOSINE METHYLATION, REFERENCE GENOME, ARABIDOPSIS, N-6-METHYLADENOSINE, SEQUENCE, METTL16, METHYLTRANSFERASE, TRANSCRIPTION",
author = "Parker, {Matthew T.} and Soanes, {Beth K.} and Jelena Kusakina and Antoine Larrieu and Katarzyna Knop and Nisha Joy and Friedrich Breidenbach and Anna Sherwood and Barton, {Geoffrey J.} and Fica, {Sebastian M.} and Davies, {Brendan H.} and Simpson, {Gordon G.}",
year = "2022",
doi = "10.7554/eLife.78808",
language = "English",
volume = "11",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site

AU - Parker, Matthew T.

AU - Soanes, Beth K.

AU - Kusakina, Jelena

AU - Larrieu, Antoine

AU - Knop, Katarzyna

AU - Joy, Nisha

AU - Breidenbach, Friedrich

AU - Sherwood, Anna

AU - Barton, Geoffrey J.

AU - Fica, Sebastian M.

AU - Davies, Brendan H.

AU - Simpson, Gordon G.

PY - 2022

Y1 - 2022

N2 - Alternative splicing of messenger RNAs is associated with the evolution of developmentally complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA 5' splice site into the spliceosome active site depends upon pairing with the conserved ACAGA sequence of U6 snRNA. In some species, including humans, the central adenosine of the ACAGA box is modified by N-6 methylation, but the role of this m(6)A modification is poorly understood. Here, we show that m(6)A modified U6 snRNA determines the accuracy and efficiency of splicing. We reveal that the conserved methyltransferase, FIONA1, is required for Arabidopsis U6 snRNA m(6)A modification. Arabidopsis fio1 mutants show disrupted patterns of splicing that can be explained by the sequence composition of 5' splice sites and cooperative roles for U5 and U6 snRNA in splice site selection. U6 snRNA m(6)A influences 3' splice site usage. We generalise these findings to reveal two major classes of 5' splice site in diverse eukaryotes, which display anti-correlated interaction potential with U5 snRNA loop 1 and the U6 snRNA ACAGA box. We conclude that U6 snRNA m(6)A modification contributes to the selection of degenerate 5' splice sites crucial to alternative splicing.

AB - Alternative splicing of messenger RNAs is associated with the evolution of developmentally complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA 5' splice site into the spliceosome active site depends upon pairing with the conserved ACAGA sequence of U6 snRNA. In some species, including humans, the central adenosine of the ACAGA box is modified by N-6 methylation, but the role of this m(6)A modification is poorly understood. Here, we show that m(6)A modified U6 snRNA determines the accuracy and efficiency of splicing. We reveal that the conserved methyltransferase, FIONA1, is required for Arabidopsis U6 snRNA m(6)A modification. Arabidopsis fio1 mutants show disrupted patterns of splicing that can be explained by the sequence composition of 5' splice sites and cooperative roles for U5 and U6 snRNA in splice site selection. U6 snRNA m(6)A influences 3' splice site usage. We generalise these findings to reveal two major classes of 5' splice site in diverse eukaryotes, which display anti-correlated interaction potential with U5 snRNA loop 1 and the U6 snRNA ACAGA box. We conclude that U6 snRNA m(6)A modification contributes to the selection of degenerate 5' splice sites crucial to alternative splicing.

KW - splicing

KW - epitranscriptome

KW - m6A

KW - flowering time

KW - ambient temperature

KW - A

KW - thaliana

KW - C

KW - elegans

KW - D

KW - melanogaster

KW - Human

KW - Zebrafish

KW - SMALL NUCLEAR RNAS

KW - CRYO-EM STRUCTURE

KW - N-6-ADENOSINE METHYLATION

KW - REFERENCE GENOME

KW - ARABIDOPSIS

KW - N-6-METHYLADENOSINE

KW - SEQUENCE

KW - METTL16

KW - METHYLTRANSFERASE

KW - TRANSCRIPTION

U2 - 10.7554/eLife.78808

DO - 10.7554/eLife.78808

M3 - Journal article

C2 - 36409063

VL - 11

JO - eLife

JF - eLife

SN - 2050-084X

M1 - 78808

ER -

ID: 338533197