Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow. / Li, Hongzhe; Ghazanfari, Roshanak; Zacharaki, Dimitra; Ditzel, Nicholas; Isern, Joan; Ekblom, Marja; Méndez-Ferrer, Simón; Kassem, Moustapha; Scheding, Stefan.

I: Stem Cell Reports, Bind 3, Nr. 6, 09.12.2014, s. 965-74.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Li, H, Ghazanfari, R, Zacharaki, D, Ditzel, N, Isern, J, Ekblom, M, Méndez-Ferrer, S, Kassem, M & Scheding, S 2014, 'Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow', Stem Cell Reports, bind 3, nr. 6, s. 965-74. https://doi.org/10.1016/j.stemcr.2014.09.018

APA

Li, H., Ghazanfari, R., Zacharaki, D., Ditzel, N., Isern, J., Ekblom, M., Méndez-Ferrer, S., Kassem, M., & Scheding, S. (2014). Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow. Stem Cell Reports, 3(6), 965-74. https://doi.org/10.1016/j.stemcr.2014.09.018

Vancouver

Li H, Ghazanfari R, Zacharaki D, Ditzel N, Isern J, Ekblom M o.a. Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow. Stem Cell Reports. 2014 dec. 9;3(6):965-74. https://doi.org/10.1016/j.stemcr.2014.09.018

Author

Li, Hongzhe ; Ghazanfari, Roshanak ; Zacharaki, Dimitra ; Ditzel, Nicholas ; Isern, Joan ; Ekblom, Marja ; Méndez-Ferrer, Simón ; Kassem, Moustapha ; Scheding, Stefan. / Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow. I: Stem Cell Reports. 2014 ; Bind 3, Nr. 6. s. 965-74.

Bibtex

@article{3c57ccd1997d4222851bf238e209fa46,

title = "Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow",

abstract = "Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.",

author = "Hongzhe Li and Roshanak Ghazanfari and Dimitra Zacharaki and Nicholas Ditzel and Joan Isern and Marja Ekblom and Sim{\'o}n M{\'e}ndez-Ferrer and Moustapha Kassem and Stefan Scheding",

year = "2014",

month = dec,

day = "9",

doi = "10.1016/j.stemcr.2014.09.018",

language = "English",

volume = "3",

pages = "965--74",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "6",

}

RIS

TY - JOUR

T1 - Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow

AU - Li, Hongzhe

AU - Ghazanfari, Roshanak

AU - Zacharaki, Dimitra

AU - Ditzel, Nicholas

AU - Isern, Joan

AU - Ekblom, Marja

AU - Méndez-Ferrer, Simón

AU - Kassem, Moustapha

AU - Scheding, Stefan

PY - 2014/12/9

Y1 - 2014/12/9

N2 - Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.

AB - Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.

U2 - 10.1016/j.stemcr.2014.09.018

DO - 10.1016/j.stemcr.2014.09.018

M3 - Journal article

C2 - 25454633

VL - 3

SP - 965

EP - 974

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 6

ER -

ID: 129742282