Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise
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Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise. / Mikkelsen, U R; Langberg, H; Helmark, I C; Skovgaard, D; Andersen, L L; Kjaer, M; Mackey, Abigail.
I: Journal of Applied Physiology, Bind 107, Nr. 5, 11.2009, s. 1600-1611.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise
AU - Mikkelsen, U R
AU - Langberg, H
AU - Helmark, I C
AU - Skovgaard, D
AU - Andersen, L L
AU - Kjaer, M
AU - Mackey, Abigail
PY - 2009/11
Y1 - 2009/11
N2 - Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.
AB - Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentric contractions with each leg. An NSAID was infused via a microdialysis catheter into the vastus lateralis muscle of one leg (NSAID leg) before, during, and for 4.5 h after exercise, with the other leg working as a control (unblocked leg). Muscle biopsies were collected before and 8 days after exercise. Changes in satellite cells and inflammatory cell numbers were investigated by immunohistochemistry. Satellite cells were identified using antibodies against neural cell adhesion molecule and Pax7. The number of Pax7(+) cells per myofiber was increased by 96% on day 8 after exercise in the unblocked leg (0.14 +/- 0.04, mean +/- SE) compared with the prevalue (0.07 +/- 0.02, P < 0.05), whereas the number of Pax7(+) cells was unchanged in the leg muscles exposed to the NSAID (0.07 +/- 0.01). The number of inflammatory cells (CD68(+) or CD16(+) cells) was not significantly increased in either of the legs 8 days after exercise and was unaffected by the NSAID. The main finding in the present study was that the NSAID infusion for 7.5 h during the exercise day suppressed the exercise-induced increase in the number of satellite cells 8 days after exercise. These results suggest that NSAIDs negatively affect satellite cell activity after unaccustomed eccentric exercise.
KW - Adult
KW - Anti-Inflammatory Agents, Non-Steroidal
KW - Cell Proliferation
KW - Cytokines
KW - Humans
KW - Indomethacin
KW - Infusions, Parenteral
KW - Injections, Intramuscular
KW - Male
KW - Muscle, Skeletal
KW - Physical Endurance
KW - Physical Exertion
KW - Satellite Cells, Skeletal Muscle
KW - Young Adult
U2 - 10.1152/japplphysiol.00707.2009
DO - 10.1152/japplphysiol.00707.2009
M3 - Journal article
C2 - 19713429
VL - 107
SP - 1600
EP - 1611
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 5
ER -
ID: 123222770