Lipocalin 2 is protective against E. coli pneumonia
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Lipocalin 2 is protective against E. coli pneumonia. / Wu, Hong; Santoni-Rugiu, Eric; Ralfkiaer, Elisabeth; Porse, Bo T; Moser, Claus; Høiby, Niels; Borregaard, Niels; Cowland, Jack B; Wu, Hong; Santoni-Rugiu, Eric; Ralfkiær, Elisabeth; Porse, Bo Torben; Moser, Claus Ernst; Høiby, Niels; Borregaard, Niels; Cowland, Jack.
I: Respiratory research (Online), Bind 11, Nr. 96, 15.06.2010.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Lipocalin 2 is protective against E. coli pneumonia
AU - Wu, Hong
AU - Santoni-Rugiu, Eric
AU - Ralfkiaer, Elisabeth
AU - Porse, Bo T
AU - Moser, Claus
AU - Høiby, Niels
AU - Borregaard, Niels
AU - Cowland, Jack B
AU - Wu, Hong
AU - Santoni-Rugiu, Eric
AU - Ralfkiær, Elisabeth
AU - Porse, Bo Torben
AU - Moser, Claus Ernst
AU - Høiby, Niels
AU - Borregaard, Niels
AU - Cowland, Jack
PY - 2010/6/15
Y1 - 2010/6/15
N2 - BACKGROUND: Lipocalin 2 is a bacteriostatic protein that binds the siderophore enterobactin, an iron-chelating molecule produced by Escherichia coli (E. coli) that is required for bacterial growth. Infection of the lungs by E. coli is rare despite a frequent exposure to this commensal bacterium. Lipocalin 2 is an effector molecule of the innate immune system and could therefore play a role in hindering growth of E. coli in the lungs. METHODS: Lipocalin 2 knock-out and wild type mice were infected with two strains of E. coli. The lungs were removed 48 hours post-infection and examined for lipocalin 2 and MMP9 (a myeloid marker protein) by immunohistochemical staining and western blotting. Bacterial numbers were assessed in the lungs of the mice at 2 and 5 days after infection and mortality of the mice was monitored over a five-day period. The effect of administering ferrichrome (an iron source that cannot be bound by lipocalin 2) along with E.coli was also examined. RESULTS: Intratracheal installation of E. coli in mice resulted in strong induction of lipocalin 2 expression in bronchial epithelium and alveolar type II pneumocytes. Migration of myeloid cells to the site of infection also contributed to an increased lipocalin 2 level in the lungs. Significant higher bacterial numbers were observed in the lungs of lipocalin 2 knock-out mice on days 2 and 5 after infection with E. coli (p < 0.05). In addition, a higher number of E. coli was found in the spleen of surviving lipocalin 2 knock-out mice on day 5 post-infection than in the corresponding wild-type mice (p < 0.05). The protective effect against E. coli infection in wild type mice could be counteracted by the siderophore ferrichrome, indicating that the protective effect of lipocalin 2 depends on its ability to sequester iron. CONCLUSIONS: Lipocalin 2 is important for protection of airways against infection by E. coli.
AB - BACKGROUND: Lipocalin 2 is a bacteriostatic protein that binds the siderophore enterobactin, an iron-chelating molecule produced by Escherichia coli (E. coli) that is required for bacterial growth. Infection of the lungs by E. coli is rare despite a frequent exposure to this commensal bacterium. Lipocalin 2 is an effector molecule of the innate immune system and could therefore play a role in hindering growth of E. coli in the lungs. METHODS: Lipocalin 2 knock-out and wild type mice were infected with two strains of E. coli. The lungs were removed 48 hours post-infection and examined for lipocalin 2 and MMP9 (a myeloid marker protein) by immunohistochemical staining and western blotting. Bacterial numbers were assessed in the lungs of the mice at 2 and 5 days after infection and mortality of the mice was monitored over a five-day period. The effect of administering ferrichrome (an iron source that cannot be bound by lipocalin 2) along with E.coli was also examined. RESULTS: Intratracheal installation of E. coli in mice resulted in strong induction of lipocalin 2 expression in bronchial epithelium and alveolar type II pneumocytes. Migration of myeloid cells to the site of infection also contributed to an increased lipocalin 2 level in the lungs. Significant higher bacterial numbers were observed in the lungs of lipocalin 2 knock-out mice on days 2 and 5 after infection with E. coli (p < 0.05). In addition, a higher number of E. coli was found in the spleen of surviving lipocalin 2 knock-out mice on day 5 post-infection than in the corresponding wild-type mice (p < 0.05). The protective effect against E. coli infection in wild type mice could be counteracted by the siderophore ferrichrome, indicating that the protective effect of lipocalin 2 depends on its ability to sequester iron. CONCLUSIONS: Lipocalin 2 is important for protection of airways against infection by E. coli.
U2 - 10.1186/1465-9921-11-96
DO - 10.1186/1465-9921-11-96
M3 - Journal article
C2 - 20633248
VL - 11
JO - Respiratory Research (Print)
JF - Respiratory Research (Print)
SN - 1465-9921
IS - 96
ER -
ID: 21661619