Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing
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Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing. / Ibrahim, M E; Hag-Ali, M; el-Hassan, A M; Theander, T G; Kharazmi, A.
I: Parasitology Reseach, Bind 80, Nr. 7, 1994, s. 569-74.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing
AU - Ibrahim, M E
AU - Hag-Ali, M
AU - el-Hassan, A M
AU - Theander, T G
AU - Kharazmi, A
N1 - Keywords: Animals; Antimony Sodium Gluconate; Cell Line; Drug Resistance; Humans; Leishmania donovani; Leishmania major; Leishmaniasis; Leishmaniasis, Cutaneous; Leishmaniasis, Visceral; Macrophages; Mice; Sudan
PY - 1994
Y1 - 1994
N2 - A total of 17 Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain of L. major was adapted to grow at a high concentration of Pentostam (1000 micrograms/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20 micrograms/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite.
AB - A total of 17 Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain of L. major was adapted to grow at a high concentration of Pentostam (1000 micrograms/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20 micrograms/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite.
M3 - Journal article
C2 - 7855121
VL - 80
SP - 569
EP - 574
JO - Parasitology Research
JF - Parasitology Research
SN - 0932-0113
IS - 7
ER -
ID: 6766708