Isolation of a substance activating foot formation in hydra
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Isolation of a substance activating foot formation in hydra. / Grimmelikhuijzen, C J; Schaller, H C.
I: Results and Problems in Cell Differentiation, Bind 6, Nr. 5-6, 01.12.1977, s. 297-305.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Isolation of a substance activating foot formation in hydra
AU - Grimmelikhuijzen, C J
AU - Schaller, H C
PY - 1977/12/1
Y1 - 1977/12/1
N2 - We have developed an assay for a substance from hydra that accelerates foot regeneration in the animal. This substance is specific for the foot as evidenced by the following findings: (1) It is present in the animal as a steep gradient descending from foot to head, paralleling the foot-forming potential of the tissue (2) It does not accelerate head regeneration, nor do the head factors of hydra discovered by Schaller (1973) and Berking (1977) accelerate foot regeneration. We propose that the foot-activating substance is a morphogen responsible for foot formation in hydra. The foot activator can be extracted from hydra tissue with methanol and separated from other known morphogens of hydra by gel filtration and ion-exchange chromatography. A substance with similar biological and physicochemical properties can be isolated from sea anemones.
AB - We have developed an assay for a substance from hydra that accelerates foot regeneration in the animal. This substance is specific for the foot as evidenced by the following findings: (1) It is present in the animal as a steep gradient descending from foot to head, paralleling the foot-forming potential of the tissue (2) It does not accelerate head regeneration, nor do the head factors of hydra discovered by Schaller (1973) and Berking (1977) accelerate foot regeneration. We propose that the foot-activating substance is a morphogen responsible for foot formation in hydra. The foot activator can be extracted from hydra tissue with methanol and separated from other known morphogens of hydra by gel filtration and ion-exchange chromatography. A substance with similar biological and physicochemical properties can be isolated from sea anemones.
KW - Animals
KW - Dose-Response Relationship, Drug
KW - Growth Substances
KW - Hydra
KW - Morphogenesis
KW - Regeneration
M3 - Journal article
C2 - 597869
VL - 6
SP - 297
EP - 305
JO - Results and Problems in Cell Differentiation
JF - Results and Problems in Cell Differentiation
SN - 0080-1844
IS - 5-6
ER -
ID: 33515022