TY - JOUR

T1 - Isolation and charaterization of hexasaccharides derived from heparin. Analysis by HPLC and elucidation of structure by 1H NMR

AU - Larnkjær, Anni

AU - Hansen, Svend Høime

AU - Østergaard, Per B.

N1 - (Ekstern)

PY - 1995

Y1 - 1995

N2 - Four hexasaccharides representing major structural sequences of heparin were isolated and characterized after degradation of heparin by heparinase. The structures were determined from two-dimensional 1H NMR spectroscopy including TOCSY (total correlated spectroscopy), COSY (correlated spectroscopy), and ROESY (rotating frame nuclear Overhauser enhancement spectroscopy) methods, providing new data on hexasaccharides. One of the hexasaccharides, the last eluting component from anion exchange chromatography, was derived from the tri-sulfated repeating disaccharide, α-l-idopyranosyluronic acid 2-sulfate-(1 → 4)-2-amino-2-deoxy-d-glucopyranose 6,N-disulfate, and having the structure δUA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S. The second hexasaccharide contained a nonsulfated d-glucuronic acid unit instead of the l-iduronic acid adjacent to the reducing end, and having the structure δUA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-β-d-GlcA p-(1 → 4)-α-d-GlcN p2S6S. The last two hexasaccharides were obtained in lower yield and they have not been isolated and characterized before. The structure of the third saccharide corresponded to a trimer of the repeating disaccharide except for the lack of a 6-O-sulfate group at the reducing end glucosamine residue; δUA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S-(1.

AB - Four hexasaccharides representing major structural sequences of heparin were isolated and characterized after degradation of heparin by heparinase. The structures were determined from two-dimensional 1H NMR spectroscopy including TOCSY (total correlated spectroscopy), COSY (correlated spectroscopy), and ROESY (rotating frame nuclear Overhauser enhancement spectroscopy) methods, providing new data on hexasaccharides. One of the hexasaccharides, the last eluting component from anion exchange chromatography, was derived from the tri-sulfated repeating disaccharide, α-l-idopyranosyluronic acid 2-sulfate-(1 → 4)-2-amino-2-deoxy-d-glucopyranose 6,N-disulfate, and having the structure δUA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S. The second hexasaccharide contained a nonsulfated d-glucuronic acid unit instead of the l-iduronic acid adjacent to the reducing end, and having the structure δUA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-β-d-GlcA p-(1 → 4)-α-d-GlcN p2S6S. The last two hexasaccharides were obtained in lower yield and they have not been isolated and characterized before. The structure of the third saccharide corresponded to a trimer of the repeating disaccharide except for the lack of a 6-O-sulfate group at the reducing end glucosamine residue; δUA p2S-(1 → 4)-α-d-GlcN p2S6S-(1 → 4)-α-l-IdoA p2S-(1 → 4)-α-d-GlcN p2S6S-(1.

UR - http://www.scopus.com/inward/record.url?scp=0028978387&partnerID=8YFLogxK

U2 - 10.1016/0008-6215(94)00247-D

DO - 10.1016/0008-6215(94)00247-D

M3 - Journal article

C2 - 7697649

AN - SCOPUS:0028978387

VL - 266

SP - 37

EP - 52

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

IS - 1

ER -