Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA
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Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA. / Jensen, Kaj Frank; Nes, Ingolf F.; Wells, Robert D.
I: Nucleic Acids Research, Bind 3, Nr. 11, 1976, s. 3143-3156.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA
AU - Jensen, Kaj Frank
AU - Nes, Ingolf F.
AU - Wells, Robert D.
PY - 1976
Y1 - 1976
N2 - Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100–200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of 80 placDNA and 80 plac DNA containing the LI deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear plac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNAs revealed the absence of localized single-stranded regions.
AB - Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100–200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of 80 placDNA and 80 plac DNA containing the LI deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear plac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNAs revealed the absence of localized single-stranded regions.
M3 - Journal article
VL - 3
SP - 3143
EP - 3156
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 11
ER -
ID: 1393028