TY - JOUR

T1 - Interleukin-6 production by thyroid epithelial cells. Enhancement by interleukin-1

AU - Diamant, Marcus

AU - Kayser, Lars

AU - Rasmussen, Åse Krogh

AU - Bech, Karine

AU - Feldt-Rassmussen, Ulla

N1 - Funding Information: We thank Dr Steven Gillis, Dr Lucien Aarden, Dr P. Ealey and Dr G.R. Adolf for generously providing the cytokines, antibodies and cell lines used and the Novo-Nordisk for providing human insulin. This study was supported by grants from the Danish Hospital Foundation for Medical Research, Region of Copenhagen, the Faroe Islands and Greenland, The foundation of managing director Jacob Madsen and wife Olga Madsen, The Danish Medical Research Council, the Danish Biotechnology Program, The Foundation for the Advancement of Medical science, and the Danish Research Academy.

PY - 1991

Y1 - 1991

N2 - Interleukin-1 is a potent inhibitor of thyroglobulin and cAMP production in human thyroid cells and the inhibitory effect is enhanced by tumor necrosis factor-α and interferon-β In the present study secondary cultures of human thyroid cells produced interleukin-6 and the production was significantly increased after exposure of the cells to recombinant interleukin-α and -1β This increase was dose-dependent and concomitant of the IL-1 induced decrease in cAMP and thyroglobulin production. Both tumor necrosis factor-α and -β also augmented interleukin-6 production, but less potently than interleukin-1. Interferon-γ did not affect the production of interleukin-6. The rat thyroid cell line FRTL-5 produced interleukin-6 spontaneously, and the production was enhanced after addition of recombinant interleukin-1β. A pathogenetic role of interleukin-6 in autoimmune thyroid disease is suggested.

AB - Interleukin-1 is a potent inhibitor of thyroglobulin and cAMP production in human thyroid cells and the inhibitory effect is enhanced by tumor necrosis factor-α and interferon-β In the present study secondary cultures of human thyroid cells produced interleukin-6 and the production was significantly increased after exposure of the cells to recombinant interleukin-α and -1β This increase was dose-dependent and concomitant of the IL-1 induced decrease in cAMP and thyroglobulin production. Both tumor necrosis factor-α and -β also augmented interleukin-6 production, but less potently than interleukin-1. Interferon-γ did not affect the production of interleukin-6. The rat thyroid cell line FRTL-5 produced interleukin-6 spontaneously, and the production was enhanced after addition of recombinant interleukin-1β. A pathogenetic role of interleukin-6 in autoimmune thyroid disease is suggested.

KW - Cytokines

KW - Human thyroid cells

KW - Interleukin-1

KW - Interleukin-6

KW - Rat thyroid cell line

UR - http://www.scopus.com/inward/record.url?scp=0026322692&partnerID=8YFLogxK

U2 - 10.3109/08916939108994704

DO - 10.3109/08916939108994704

M3 - Journal article

C2 - 1812993

AN - SCOPUS:0026322692

VL - 11

SP - 21

EP - 26

JO - Autoimmunity

JF - Autoimmunity

SN - 0891-6934

IS - 1

ER -