Interferon-¿ production by human T cells and natural killer cells in vitro in response to antigens from the two intracellular pathogens Mycobacterium tuberculosis and Leishmania major
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Interferon-¿ production by human T cells and natural killer cells in vitro in response to antigens from the two intracellular pathogens Mycobacterium tuberculosis and Leishmania major. / Kemp, K; Hviid, L; Kharazmi, A; Kemp, M.
I: Scandinavian Journal of Immunology, Bind 46, Nr. 5, 1997, s. 495-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Interferon-¿ production by human T cells and natural killer cells in vitro in response to antigens from the two intracellular pathogens Mycobacterium tuberculosis and Leishmania major
AU - Kemp, K
AU - Hviid, L
AU - Kharazmi, A
AU - Kemp, M
N1 - Keywords: Adult; Animals; Antigens, Bacterial; Antigens, CD3; Antigens, CD56; Antigens, Protozoan; BCG Vaccine; Cells, Cultured; Humans; Immunization; Interferon Type II; Killer Cells, Natural; Leishmania major; Lymphocyte Activation; Mycobacterium tuberculosis; T-Lymphocytes; Th1 Cells; Tuberculin
PY - 1997
Y1 - 1997
N2 - Acquired resistance to both mycobacteria and Leishmania is primarily mediated by interferon-gamma (IFN-gamma), which triggers mechanisms leading to the death of the microorganism in macrophages. In this study, cell activation and IFN-gamma production was investigated in human peripheral blood mononuclear cells (PBMC) from individuals previously sensitized to tuberculin and without known exposure to Leishmania parasites. Immune staining for intracellular IFN-gamma and surface markers allowed flow cytometric identification of the cellular sources of IFN-gamma in cell cultures incubated with purified protein derivative of tuberculin (PPD) and Leishmania antigens. It was found that IFN-gamma was produced in response to both PPD and Leishmania stimulant by T cells in the cultures. Activation of IFN-gamma producing natural killer (NK) cells was demonstrated only in some cultures, and only with concomitant T cell activation.
AB - Acquired resistance to both mycobacteria and Leishmania is primarily mediated by interferon-gamma (IFN-gamma), which triggers mechanisms leading to the death of the microorganism in macrophages. In this study, cell activation and IFN-gamma production was investigated in human peripheral blood mononuclear cells (PBMC) from individuals previously sensitized to tuberculin and without known exposure to Leishmania parasites. Immune staining for intracellular IFN-gamma and surface markers allowed flow cytometric identification of the cellular sources of IFN-gamma in cell cultures incubated with purified protein derivative of tuberculin (PPD) and Leishmania antigens. It was found that IFN-gamma was produced in response to both PPD and Leishmania stimulant by T cells in the cultures. Activation of IFN-gamma producing natural killer (NK) cells was demonstrated only in some cultures, and only with concomitant T cell activation.
U2 - 10.1046/j.1365-3083.1997.d01-154.x
DO - 10.1046/j.1365-3083.1997.d01-154.x
M3 - Journal article
C2 - 9393632
VL - 46
SP - 495
EP - 499
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 5
ER -
ID: 6748150