Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture
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Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture. / Tominski, Claudia; Lösekann-Behrens, Tina; Ruecker, Alexander; Hagemann, Nikolas; Kleindienst, Sara; Mueller, Carsten W.; Höschen, Carmen; Kögel-Knabner, Ingrid; Kappler, Andreas; Behrens, Sebastian.
I: Applied and Environmental Microbiology, Bind 84, Nr. 9, e02166-17, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture
AU - Tominski, Claudia
AU - Lösekann-Behrens, Tina
AU - Ruecker, Alexander
AU - Hagemann, Nikolas
AU - Kleindienst, Sara
AU - Mueller, Carsten W.
AU - Höschen, Carmen
AU - Kögel-Knabner, Ingrid
AU - Kappler, Andreas
AU - Behrens, Sebastian
PY - 2018
Y1 - 2018
N2 - The enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter deposition fluorescence in situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, singlecell activities, and interactions among the two most abundant microbial community members (i.e., Gallionellaceae sp. and Bradyrhizobium spp.) under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts showed the dominance of the Fe(II) oxidizer Gallionellaceae sp. under autotrophic conditions as well as of Bradyrhizobium spp. under heterotrophic conditions. We used NanoSIMS to monitor the fate of 13C-labeled bicarbonate and acetate as well as 15N-labeled ammonium at the single-cell level for both taxa. Under autotrophic conditions, only the Gallionellaceae sp. was actively incorporating 13C-labeled bicarbonate and 15N-labeled ammonium. Interestingly, both Bradyrhizobium spp. and Gallionellaceae sp. became enriched in [13C]acetate and [15N]ammonium under heterotrophic conditions. Our experiments demonstrated that Gallionellaceae sp. was capable of assimilating [13C]acetate while Bradyrhizobium spp. were not able to fix CO2, although a metagenomics survey of culture KS recently revealed that Gallionellaceae sp. lacks genes for acetate uptake and that the Bradyrhizobium sp. carries the genetic potential to fix CO2. The study furthermore extends our understanding of the microbial reactions that interlink the nitrogen and Fe cycles in the environment.
AB - The enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter deposition fluorescence in situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, singlecell activities, and interactions among the two most abundant microbial community members (i.e., Gallionellaceae sp. and Bradyrhizobium spp.) under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts showed the dominance of the Fe(II) oxidizer Gallionellaceae sp. under autotrophic conditions as well as of Bradyrhizobium spp. under heterotrophic conditions. We used NanoSIMS to monitor the fate of 13C-labeled bicarbonate and acetate as well as 15N-labeled ammonium at the single-cell level for both taxa. Under autotrophic conditions, only the Gallionellaceae sp. was actively incorporating 13C-labeled bicarbonate and 15N-labeled ammonium. Interestingly, both Bradyrhizobium spp. and Gallionellaceae sp. became enriched in [13C]acetate and [15N]ammonium under heterotrophic conditions. Our experiments demonstrated that Gallionellaceae sp. was capable of assimilating [13C]acetate while Bradyrhizobium spp. were not able to fix CO2, although a metagenomics survey of culture KS recently revealed that Gallionellaceae sp. lacks genes for acetate uptake and that the Bradyrhizobium sp. carries the genetic potential to fix CO2. The study furthermore extends our understanding of the microbial reactions that interlink the nitrogen and Fe cycles in the environment.
KW - Bradyrhizobium
KW - Chemolithoautotrophic ferrous iron [Fe(II)] oxidation
KW - Fluorescence in situ hybridization (FISH)
KW - Gallionellaceae
KW - Nanoscale secondary-ion mass spectrometry (NanoSIMS)
KW - Neutrophilic
KW - Nitrate-dependent Fe(II) oxidation (NDFO)
U2 - 10.1128/AEM.02166-17
DO - 10.1128/AEM.02166-17
M3 - Journal article
C2 - 29500258
AN - SCOPUS:85045506772
VL - 84
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 9
M1 - e02166-17
ER -
ID: 238951830