Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile
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Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile. / Amlacher, Stefan; Sarges, Phillip; Flemming, Dirk; van Noort, Vera; Kunze, Ruth; Devos, Damien P; Arumugam, Manimozhiyan; Bork, Peer; Hurt, Ed.
I: Cell, Bind 146, Nr. 2, 2011, s. 277-89.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile
AU - Amlacher, Stefan
AU - Sarges, Phillip
AU - Flemming, Dirk
AU - van Noort, Vera
AU - Kunze, Ruth
AU - Devos, Damien P
AU - Arumugam, Manimozhiyan
AU - Bork, Peer
AU - Hurt, Ed
N1 - Copyright © 2011 Elsevier Inc. All rights reserved.
PY - 2011
Y1 - 2011
N2 - Despite decades of research, the structure and assembly of the nuclear pore complex (NPC), which is composed of ~30 nucleoporins (Nups), remain elusive. Here, we report the genome of the thermophilic fungus Chaetomium thermophilum (ct) and identify the complete repertoire of Nups therein. The thermophilic proteins show improved properties for structural and biochemical studies compared to their mesophilic counterparts, and purified ctNups enabled the reconstitution of the inner pore ring module that spans the width of the NPC from the anchoring membrane to the central transport channel. This module is composed of two large Nups, Nup192 and Nup170, which are flexibly bridged by short linear motifs made up of linker Nups, Nic96 and Nup53. This assembly illustrates how Nup interactions can generate structural plasticity within the NPC scaffold. Our findings therefore demonstrate the utility of the genome of a thermophilic eukaryote for studying complex molecular machines.
AB - Despite decades of research, the structure and assembly of the nuclear pore complex (NPC), which is composed of ~30 nucleoporins (Nups), remain elusive. Here, we report the genome of the thermophilic fungus Chaetomium thermophilum (ct) and identify the complete repertoire of Nups therein. The thermophilic proteins show improved properties for structural and biochemical studies compared to their mesophilic counterparts, and purified ctNups enabled the reconstitution of the inner pore ring module that spans the width of the NPC from the anchoring membrane to the central transport channel. This module is composed of two large Nups, Nup192 and Nup170, which are flexibly bridged by short linear motifs made up of linker Nups, Nic96 and Nup53. This assembly illustrates how Nup interactions can generate structural plasticity within the NPC scaffold. Our findings therefore demonstrate the utility of the genome of a thermophilic eukaryote for studying complex molecular machines.
KW - Amino Acid Sequence
KW - Chaetomium
KW - Fungal Proteins
KW - Genome, Fungal
KW - Molecular Sequence Data
KW - Nuclear Pore
KW - Nuclear Pore Complex Proteins
KW - Phylogeny
KW - Sequence Alignment
KW - Two-Hybrid System Techniques
U2 - 10.1016/j.cell.2011.06.039
DO - 10.1016/j.cell.2011.06.039
M3 - Journal article
C2 - 21784248
VL - 146
SP - 277
EP - 289
JO - Cell
JF - Cell
SN - 0092-8674
IS - 2
ER -
ID: 43975764