Ingestible Device for Gastric Fluid Sampling
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The composition of the human gastrointestinal microbiota is linked to the health of the host, and interventions targeting intestinal microbes may thus be designed to prevent or mitigate disease. As the spatiotemporal structure and physiology impact the residing bacterial community, local sampling is gaining attention, with various ingestible sampling devices being developed to target specific sites. However, the stomach has received limited attention, despite its potential downstream influence. This work presents a simple ingestible device for gastric fluid sampling and outlines a series of characterizations to ensure device safety, reliability, and accuracy. In vitro testing determined seal effectiveness, mechanical integrity, biocompatibility, and device-sample inertness. In situ and ex vivo testing confirmed sampling accuracy, demonstrated microbiome composition stability for at least 24 h, and differentiation of microbiota between two primates. 16S rRNA gene amplicon sequencing of samples from a porcine ingestion model showed that samples resembled post-mortem gastric samples and differed from fecal and colonic samples. Also addressed in this study, is production scalability and shelf-life to facilitate the safe and effective deployment of devices in clinical settings.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Advanced Materials Technologies |
| Antal sider | 14 |
| ISSN | 2365-709X |
| DOI | |
| Status | E-pub ahead of print - 2024 |
Bibliografisk note
Funding Information:
The authors would like to acknowledge the Danish National Research Foundation (DNRF) (Grant No. DNRF122), Villum Fonden (Grant No. 9301) for intelligent drug delivery and sensing using microcontainers and nanomechanics (IDUN), Novo Nordisk Foundation (NNF) for EMGUT\u2010Energy Materials for the GUT (Grant No. NNF22OC0072961), and NNF PRIMA (Grant No. NNF19OC0056246). N.K.M. was supported by an Excellence Ph.D. Scholarship from DTU Health Tech and currently an Internationalization Fellowship from Carlsberg Foundation (Grant No. CF21\u20100614). Infrastructure at the Center for Evolutionary Hologenomics was funded by the DNRF grant DNRF143. The authors would like to express their gratitude to Pi Bondegaard (Technical University of Denmark) for support with the bacteria leakage experiments, Dr. Xiaohoa Michelle Ching (Harvard University) for preparing Figure 3a , Myka Mae Campo Duran (Technical University of Denmark) for preparing pig sketch in Figure 3e , Copenhagen Zoo for giving access to euthanized lemurs and baboons, Aakil Lalwani (Technical University of Denmark) for support with CAD design and 3D printing, and Andr\u00E1s Vujovits (Technical University of Denmark) for assisting with the fabrication of the trigger tool, and Dr. Maria Svendsmark Hansen (Technical University of Denmark) for proofreading the manuscript.
Publisher Copyright:
© 2024 The Author(s). Advanced Materials Technologies published by Wiley-VCH GmbH.
ID: 395142012