Ingestible Device for Gastric Fluid Sampling
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Ingestible Device for Gastric Fluid Sampling. / Mandsberg, Nikolaj Kofoed; Moro, Gafaru; Ghavami, Mahdi; Andersen, Sandra B.; de Visser, Eva Nedergaard; Bertelsen, Mads Frost; Mortensen, Martin Steen; Licht, Tine Rask; Boisen, Anja.
I: Advanced Materials Technologies, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Ingestible Device for Gastric Fluid Sampling
AU - Mandsberg, Nikolaj Kofoed
AU - Moro, Gafaru
AU - Ghavami, Mahdi
AU - Andersen, Sandra B.
AU - de Visser, Eva Nedergaard
AU - Bertelsen, Mads Frost
AU - Mortensen, Martin Steen
AU - Licht, Tine Rask
AU - Boisen, Anja
N1 - Publisher Copyright: © 2024 The Author(s). Advanced Materials Technologies published by Wiley-VCH GmbH.
PY - 2024
Y1 - 2024
N2 - The composition of the human gastrointestinal microbiota is linked to the health of the host, and interventions targeting intestinal microbes may thus be designed to prevent or mitigate disease. As the spatiotemporal structure and physiology impact the residing bacterial community, local sampling is gaining attention, with various ingestible sampling devices being developed to target specific sites. However, the stomach has received limited attention, despite its potential downstream influence. This work presents a simple ingestible device for gastric fluid sampling and outlines a series of characterizations to ensure device safety, reliability, and accuracy. In vitro testing determined seal effectiveness, mechanical integrity, biocompatibility, and device-sample inertness. In situ and ex vivo testing confirmed sampling accuracy, demonstrated microbiome composition stability for at least 24 h, and differentiation of microbiota between two primates. 16S rRNA gene amplicon sequencing of samples from a porcine ingestion model showed that samples resembled post-mortem gastric samples and differed from fecal and colonic samples. Also addressed in this study, is production scalability and shelf-life to facilitate the safe and effective deployment of devices in clinical settings.
AB - The composition of the human gastrointestinal microbiota is linked to the health of the host, and interventions targeting intestinal microbes may thus be designed to prevent or mitigate disease. As the spatiotemporal structure and physiology impact the residing bacterial community, local sampling is gaining attention, with various ingestible sampling devices being developed to target specific sites. However, the stomach has received limited attention, despite its potential downstream influence. This work presents a simple ingestible device for gastric fluid sampling and outlines a series of characterizations to ensure device safety, reliability, and accuracy. In vitro testing determined seal effectiveness, mechanical integrity, biocompatibility, and device-sample inertness. In situ and ex vivo testing confirmed sampling accuracy, demonstrated microbiome composition stability for at least 24 h, and differentiation of microbiota between two primates. 16S rRNA gene amplicon sequencing of samples from a porcine ingestion model showed that samples resembled post-mortem gastric samples and differed from fecal and colonic samples. Also addressed in this study, is production scalability and shelf-life to facilitate the safe and effective deployment of devices in clinical settings.
KW - gastric disease
KW - gut microbiome
KW - gut sampling
KW - H. pylori
KW - ingestible device
U2 - 10.1002/admt.202400434
DO - 10.1002/admt.202400434
M3 - Journal article
AN - SCOPUS:85194548466
JO - Advanced Materials Technologies
JF - Advanced Materials Technologies
SN - 2365-709X
ER -
ID: 395142012