TY - JOUR

T1 - Ingestible Device for Gastric Fluid Sampling

AU - Mandsberg, Nikolaj Kofoed

AU - Moro, Gafaru

AU - Ghavami, Mahdi

AU - Andersen, Sandra B.

AU - de Visser, Eva Nedergaard

AU - Bertelsen, Mads Frost

AU - Mortensen, Martin Steen

AU - Licht, Tine Rask

AU - Boisen, Anja

N1 - Publisher Copyright: © 2024 The Author(s). Advanced Materials Technologies published by Wiley-VCH GmbH.

PY - 2024

Y1 - 2024

N2 - The composition of the human gastrointestinal microbiota is linked to the health of the host, and interventions targeting intestinal microbes may thus be designed to prevent or mitigate disease. As the spatiotemporal structure and physiology impact the residing bacterial community, local sampling is gaining attention, with various ingestible sampling devices being developed to target specific sites. However, the stomach has received limited attention, despite its potential downstream influence. This work presents a simple ingestible device for gastric fluid sampling and outlines a series of characterizations to ensure device safety, reliability, and accuracy. In vitro testing determined seal effectiveness, mechanical integrity, biocompatibility, and device-sample inertness. In situ and ex vivo testing confirmed sampling accuracy, demonstrated microbiome composition stability for at least 24 h, and differentiation of microbiota between two primates. 16S rRNA gene amplicon sequencing of samples from a porcine ingestion model showed that samples resembled post-mortem gastric samples and differed from fecal and colonic samples. Also addressed in this study, is production scalability and shelf-life to facilitate the safe and effective deployment of devices in clinical settings.

AB - The composition of the human gastrointestinal microbiota is linked to the health of the host, and interventions targeting intestinal microbes may thus be designed to prevent or mitigate disease. As the spatiotemporal structure and physiology impact the residing bacterial community, local sampling is gaining attention, with various ingestible sampling devices being developed to target specific sites. However, the stomach has received limited attention, despite its potential downstream influence. This work presents a simple ingestible device for gastric fluid sampling and outlines a series of characterizations to ensure device safety, reliability, and accuracy. In vitro testing determined seal effectiveness, mechanical integrity, biocompatibility, and device-sample inertness. In situ and ex vivo testing confirmed sampling accuracy, demonstrated microbiome composition stability for at least 24 h, and differentiation of microbiota between two primates. 16S rRNA gene amplicon sequencing of samples from a porcine ingestion model showed that samples resembled post-mortem gastric samples and differed from fecal and colonic samples. Also addressed in this study, is production scalability and shelf-life to facilitate the safe and effective deployment of devices in clinical settings.

KW - gastric disease

KW - gut microbiome

KW - gut sampling

KW - H. pylori

KW - ingestible device

U2 - 10.1002/admt.202400434

DO - 10.1002/admt.202400434

M3 - Journal article

AN - SCOPUS:85194548466

JO - Advanced Materials Technologies

JF - Advanced Materials Technologies

SN - 2365-709X

ER -