In-gel digestion for mass spectrometric characterization of proteins and proteomes
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In-gel digestion for mass spectrometric characterization of proteins and proteomes. / Shevchenko, Andrej; Tomas, Henrik; Havlis, Jan; Olsen, Jesper Velgaard; Mann, Matthias.
I: Nature Protocols (Print Edition), Bind 1, Nr. 6, 2006, s. 2856-60.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - In-gel digestion for mass spectrometric characterization of proteins and proteomes
AU - Shevchenko, Andrej
AU - Tomas, Henrik
AU - Havlis, Jan
AU - Olsen, Jesper Velgaard
AU - Mann, Matthias
N1 - Keywords: Electrophoresis; Mass Spectrometry; Proteins; Proteomics
PY - 2006
Y1 - 2006
N2 - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
AB - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
U2 - 10.1038/nprot.2006.468
DO - 10.1038/nprot.2006.468
M3 - Journal article
C2 - 17406544
VL - 1
SP - 2856
EP - 2860
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 6
ER -
ID: 46460063