In vivo expression of a group I intron HEG from the antisense strand of Didymium ribosomal DNA
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In vivo expression of a group I intron HEG from the antisense strand of Didymium ribosomal DNA. / Johansen, Steinar D; Vader, Anna; Sjøttem, Eva; Nielsen, Henrik.
I: RNA Biology, Bind 3, Nr. 4, 2006, s. 157-62.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - In vivo expression of a group I intron HEG from the antisense strand of Didymium ribosomal DNA
AU - Johansen, Steinar D
AU - Vader, Anna
AU - Sjøttem, Eva
AU - Nielsen, Henrik
N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; DNA, Antisense; DNA, Ribosomal; Endodeoxyribonucleases; Introns; Molecular Sequence Data; Physarida
PY - 2006
Y1 - 2006
N2 - Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.
AB - Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.
M3 - Journal article
C2 - 17361110
VL - 3
SP - 157
EP - 162
JO - R N A Biology
JF - R N A Biology
SN - 1547-6286
IS - 4
ER -
ID: 14612078