Impregnation of healthy nail tissue with optical clearing agents for improved optical coherence tomography imaging
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Impregnation of healthy nail tissue with optical clearing agents for improved optical coherence tomography imaging. / Ortner, Vinzent Kevin; Mandel, Victor Desmond; Haedersdal, Merete; Philipsen, Peter Alshede.
I: Skin Research and Technology, Bind 27, Nr. 2, 2021, s. 178-182.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Impregnation of healthy nail tissue with optical clearing agents for improved optical coherence tomography imaging
AU - Ortner, Vinzent Kevin
AU - Mandel, Victor Desmond
AU - Haedersdal, Merete
AU - Philipsen, Peter Alshede
N1 - Publisher Copyright: © 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2021
Y1 - 2021
N2 - Objectives: The impact of optical tissue clearing on optical coherence tomography (OCT) for nail tissue imaging has not been investigated. This study seeks to compare the effects of an emollient and water on visualization of micromorphology and morphometric outcomes. Materials and Methods: Thirty-six healthy nail plates were processed with a fractional CO2 laser, imaged with OCT, and measured with calipers in duplicates. All samples were reassessed after 12-hour long sequential immersion in water and an emollient (Crodamol™ STS). OCT images were evaluated for thickness and scattering signal of the nail. Results: Emollient-impregnation caused stronger scatter responses (P <.0001) and decreased nail thickness (MD 45 μm, P <.0001) measured on OCT. Caliper-derived measurements were not affected by Crodamol™ (MD 11 μm, P =.5538). Hydration increased nail thickness on OCT (MD 49 μm, P <.0001) but reduced thickness measurements taken with calipers (MD 41 μm, P <.0001). Emollient-impregnation improved visualization of onychocytes compared with dry (P =.0209) and hydrated samples (P <.0001), and reduced occurrence of refractive artifacts (P <.0001). Conclusion: The use of an emollient for OCT imaging can enhance nail tissue visualization without significant effects on caliper measurements. Hydration of nails, in contrast with emollient-impregnation, may lead to disagreement between caliper- and OCT-measured nail thickness and should be practiced cautiously.
AB - Objectives: The impact of optical tissue clearing on optical coherence tomography (OCT) for nail tissue imaging has not been investigated. This study seeks to compare the effects of an emollient and water on visualization of micromorphology and morphometric outcomes. Materials and Methods: Thirty-six healthy nail plates were processed with a fractional CO2 laser, imaged with OCT, and measured with calipers in duplicates. All samples were reassessed after 12-hour long sequential immersion in water and an emollient (Crodamol™ STS). OCT images were evaluated for thickness and scattering signal of the nail. Results: Emollient-impregnation caused stronger scatter responses (P <.0001) and decreased nail thickness (MD 45 μm, P <.0001) measured on OCT. Caliper-derived measurements were not affected by Crodamol™ (MD 11 μm, P =.5538). Hydration increased nail thickness on OCT (MD 49 μm, P <.0001) but reduced thickness measurements taken with calipers (MD 41 μm, P <.0001). Emollient-impregnation improved visualization of onychocytes compared with dry (P =.0209) and hydrated samples (P <.0001), and reduced occurrence of refractive artifacts (P <.0001). Conclusion: The use of an emollient for OCT imaging can enhance nail tissue visualization without significant effects on caliper measurements. Hydration of nails, in contrast with emollient-impregnation, may lead to disagreement between caliper- and OCT-measured nail thickness and should be practiced cautiously.
KW - caliper
KW - crodamol
KW - nail tissue
KW - optical clearing agent
KW - optical coherence tomography
U2 - 10.1111/srt.12923
DO - 10.1111/srt.12923
M3 - Journal article
C2 - 32681563
AN - SCOPUS:85088129605
VL - 27
SP - 178
EP - 182
JO - Skin Research and Technology
JF - Skin Research and Technology
SN - 0909-752X
IS - 2
ER -
ID: 305024397