Impaired hapten sensitization in patients with autoimmune disease
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Impaired hapten sensitization in patients with autoimmune disease. / Bangsgaard, N; Engkilde, K; Menné, T; Løvendorf, M; Jacobsen, G K; Olsen, J; Skov, L.
I: Clinical and Experimental Immunology, Bind 165, Nr. 3, 09.2011, s. 310-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Impaired hapten sensitization in patients with autoimmune disease
AU - Bangsgaard, N
AU - Engkilde, K
AU - Menné, T
AU - Løvendorf, M
AU - Jacobsen, G K
AU - Olsen, J
AU - Skov, L
N1 - © 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology.
PY - 2011/9
Y1 - 2011/9
N2 - An inverse relation between contact allergy and autoimmune diseases is suggested from epidemiological studies. The aim of this study was to investigate susceptibility and reactivity in patients with psoriasis, patients with diabetes and healthy controls in an experimental sensitization study. We sensitized 68 adult individuals (23 patients with psoriasis, 22 patients with diabetes and 23 healthy controls) with diphenylcyclopropenone (DPCP) and assessed challenge responses with visual scoring and ultrasound. Skin biopsies from challenged skin were investigated for differences in down-regulatory mechanisms with immunohistochemistry and gene-expression profiles using microarray technology. The sensitization ratios were 26%, 36% and 65% for the psoriatic, diabetic and healthy groups, respectively. Logistic regression analysis gave an odds ratio (OR) for a patient with psoriasis or diabetes type I of being sensitized to 0·18 [95% confidence interval (CI): 0·039-0·85], P = 0·031 and 0·74 (95% CI: 0·548-1·008), P = 0·056, respectively. A high degree of forkhead box P3-positive (FoxP3(+) ) cells were found in biopsies of positively challenged reactions, but only limited numbers in negatively challenged reactions, with no difference among the groups. No specific mRNA expression was found in the challenged skin of negative elicitation reactions, also indicating no sign of active down-regulation. The study contibutes strongly to the evidence of a decreased susceptibility to develop contact allergy in individuals with autoimmune diseases such as psoriasis.
AB - An inverse relation between contact allergy and autoimmune diseases is suggested from epidemiological studies. The aim of this study was to investigate susceptibility and reactivity in patients with psoriasis, patients with diabetes and healthy controls in an experimental sensitization study. We sensitized 68 adult individuals (23 patients with psoriasis, 22 patients with diabetes and 23 healthy controls) with diphenylcyclopropenone (DPCP) and assessed challenge responses with visual scoring and ultrasound. Skin biopsies from challenged skin were investigated for differences in down-regulatory mechanisms with immunohistochemistry and gene-expression profiles using microarray technology. The sensitization ratios were 26%, 36% and 65% for the psoriatic, diabetic and healthy groups, respectively. Logistic regression analysis gave an odds ratio (OR) for a patient with psoriasis or diabetes type I of being sensitized to 0·18 [95% confidence interval (CI): 0·039-0·85], P = 0·031 and 0·74 (95% CI: 0·548-1·008), P = 0·056, respectively. A high degree of forkhead box P3-positive (FoxP3(+) ) cells were found in biopsies of positively challenged reactions, but only limited numbers in negatively challenged reactions, with no difference among the groups. No specific mRNA expression was found in the challenged skin of negative elicitation reactions, also indicating no sign of active down-regulation. The study contibutes strongly to the evidence of a decreased susceptibility to develop contact allergy in individuals with autoimmune diseases such as psoriasis.
KW - Adult
KW - Autoimmune Diseases
KW - Biopsy
KW - Cyclopropanes
KW - Dermatitis, Allergic Contact
KW - Dermis
KW - Diabetes Mellitus, Type 1
KW - Down-Regulation
KW - Epidermis
KW - Female
KW - Gene Expression
KW - Gene Expression Profiling
KW - Haptens
KW - Humans
KW - Immunization
KW - Lymphocytes
KW - Male
KW - Middle Aged
KW - Odds Ratio
KW - Oligonucleotide Array Sequence Analysis
KW - Principal Component Analysis
KW - Psoriasis
KW - Skin Tests
KW - Up-Regulation
U2 - 10.1111/j.1365-2249.2011.04428.x
DO - 10.1111/j.1365-2249.2011.04428.x
M3 - Journal article
C2 - 21668897
VL - 165
SP - 310
EP - 317
JO - Clinical and Experimental Immunology, Supplement
JF - Clinical and Experimental Immunology, Supplement
SN - 0964-2536
IS - 3
ER -
ID: 35948050