Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus
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Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus. / Theisen, M; Cox, G; Høgh, B; Jepsen, S; Vuust, J.
I: Infection and Immunity, Bind 62, Nr. 8, 08.1994, s. 3270-5.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus
AU - Theisen, M
AU - Cox, G
AU - Høgh, B
AU - Jepsen, S
AU - Vuust, J
PY - 1994/8
Y1 - 1994/8
N2 - The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.
AB - The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.
KW - Animals
KW - Base Sequence
KW - Female
KW - Humans
KW - Malaria Vaccines/immunology
KW - Mice
KW - Mice, Inbred BALB C
KW - Molecular Sequence Data
KW - Molecular Weight
KW - Plasmodium falciparum/immunology
KW - Protozoan Proteins/biosynthesis
KW - Protozoan Vaccines
KW - Vaccines, Synthetic/immunology
KW - Vaccinia virus/genetics
M3 - Journal article
C2 - 8039897
VL - 62
SP - 3270
EP - 3275
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 8
ER -
ID: 203012623