Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus

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Standard

Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus. / Theisen, M; Cox, G; Høgh, B; Jepsen, S; Vuust, J.

I: Infection and Immunity, Bind 62, Nr. 8, 08.1994, s. 3270-5.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Theisen, M, Cox, G, Høgh, B, Jepsen, S & Vuust, J 1994, 'Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus', Infection and Immunity, bind 62, nr. 8, s. 3270-5.

APA

Theisen, M., Cox, G., Høgh, B., Jepsen, S., & Vuust, J. (1994). Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus. Infection and Immunity, 62(8), 3270-5.

Vancouver

Theisen M, Cox G, Høgh B, Jepsen S, Vuust J. Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus. Infection and Immunity. 1994 aug.;62(8):3270-5.

Author

Theisen, M ; Cox, G ; Høgh, B ; Jepsen, S ; Vuust, J. / Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus. I: Infection and Immunity. 1994 ; Bind 62, Nr. 8. s. 3270-5.

Bibtex

@article{065213cc1077433ba1fdb1b4b430d274,
title = "Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus",
abstract = "The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.",
keywords = "Animals, Base Sequence, Female, Humans, Malaria Vaccines/immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Molecular Weight, Plasmodium falciparum/immunology, Protozoan Proteins/biosynthesis, Protozoan Vaccines, Vaccines, Synthetic/immunology, Vaccinia virus/genetics",
author = "M Theisen and G Cox and B H{\o}gh and S Jepsen and J Vuust",
year = "1994",
month = aug,
language = "English",
volume = "62",
pages = "3270--5",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

RIS

TY - JOUR

T1 - Immunogenicity of the Plasmodium falciparum glutamate-rich protein expressed by vaccinia virus

AU - Theisen, M

AU - Cox, G

AU - Høgh, B

AU - Jepsen, S

AU - Vuust, J

PY - 1994/8

Y1 - 1994/8

N2 - The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.

AB - The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.

KW - Animals

KW - Base Sequence

KW - Female

KW - Humans

KW - Malaria Vaccines/immunology

KW - Mice

KW - Mice, Inbred BALB C

KW - Molecular Sequence Data

KW - Molecular Weight

KW - Plasmodium falciparum/immunology

KW - Protozoan Proteins/biosynthesis

KW - Protozoan Vaccines

KW - Vaccines, Synthetic/immunology

KW - Vaccinia virus/genetics

M3 - Journal article

C2 - 8039897

VL - 62

SP - 3270

EP - 3275

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

ER -

ID: 203012623