Identification, isolation and analysis of human gut-associated lymphoid tissues

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Identification, isolation and analysis of human gut-associated lymphoid tissues. / Jørgensen, Peter B; Fenton, Thomas M; Mörbe, Urs M; Riis, Lene B; Jakobsen, Henrik L; Nielsen, Ole H; Agace, William W.

I: Nature Protocols, Bind 16, 22.02.2021, s. 2051–2067.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Jørgensen, PB, Fenton, TM, Mörbe, UM, Riis, LB, Jakobsen, HL, Nielsen, OH & Agace, WW 2021, 'Identification, isolation and analysis of human gut-associated lymphoid tissues', Nature Protocols, bind 16, s. 2051–2067. https://doi.org/10.1038/s41596-020-00482-1

APA

Jørgensen, P. B., Fenton, T. M., Mörbe, U. M., Riis, L. B., Jakobsen, H. L., Nielsen, O. H., & Agace, W. W. (2021). Identification, isolation and analysis of human gut-associated lymphoid tissues. Nature Protocols, 16, 2051–2067. https://doi.org/10.1038/s41596-020-00482-1

Vancouver

Jørgensen PB, Fenton TM, Mörbe UM, Riis LB, Jakobsen HL, Nielsen OH o.a. Identification, isolation and analysis of human gut-associated lymphoid tissues. Nature Protocols. 2021 feb. 22;16:2051–2067. https://doi.org/10.1038/s41596-020-00482-1

Author

Jørgensen, Peter B ; Fenton, Thomas M ; Mörbe, Urs M ; Riis, Lene B ; Jakobsen, Henrik L ; Nielsen, Ole H ; Agace, William W. / Identification, isolation and analysis of human gut-associated lymphoid tissues. I: Nature Protocols. 2021 ; Bind 16. s. 2051–2067.

Bibtex

@article{cce69fa871c040369b01e64f69f6ffee,
title = "Identification, isolation and analysis of human gut-associated lymphoid tissues",
abstract = "Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer's patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4-10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.",
author = "J{\o}rgensen, {Peter B} and Fenton, {Thomas M} and M{\"o}rbe, {Urs M} and Riis, {Lene B} and Jakobsen, {Henrik L} and Nielsen, {Ole H} and Agace, {William W}",
year = "2021",
month = feb,
day = "22",
doi = "10.1038/s41596-020-00482-1",
language = "English",
volume = "16",
pages = "2051–2067",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Identification, isolation and analysis of human gut-associated lymphoid tissues

AU - Jørgensen, Peter B

AU - Fenton, Thomas M

AU - Mörbe, Urs M

AU - Riis, Lene B

AU - Jakobsen, Henrik L

AU - Nielsen, Ole H

AU - Agace, William W

PY - 2021/2/22

Y1 - 2021/2/22

N2 - Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer's patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4-10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.

AB - Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer's patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4-10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.

U2 - 10.1038/s41596-020-00482-1

DO - 10.1038/s41596-020-00482-1

M3 - Journal article

C2 - 33619391

VL - 16

SP - 2051

EP - 2067

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

ER -

ID: 257322773