IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.
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Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clin. translation of differentiation protocols requires standardization of prodn. procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-assocd. protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron d. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clin. cell replacement strategies. [on SciFinder(R)]
Originalsprog | Engelsk |
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Tidsskrift | Stem Cell Reports |
Vol/bind | 9 |
Udgave nummer | 4 |
Sider (fra-til) | 1207-1220 |
Antal sider | 14 |
ISSN | 2213-6711 |
DOI | |
Status | Udgivet - okt. 2017 |
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ID: 202078010