Hypothalamic neurosecretory and circadian vasopressinergic neuronal systems in the blind cone-rod homeobox knock out mouse (Crx(-/-) ) and the 129sv wild type mouse
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Hypothalamic neurosecretory and circadian vasopressinergic neuronal systems in the blind cone-rod homeobox knock out mouse (Crx(-/-) ) and the 129sv wild type mouse. / Rovsing, Louise; Rath, Martin Fredensborg; Møller, Morten.
I: Journal of Comparative Neurology, Bind 521, Nr. 17, 20.06.2013, s. 4061-4074.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Hypothalamic neurosecretory and circadian vasopressinergic neuronal systems in the blind cone-rod homeobox knock out mouse (Crx(-/-) ) and the 129sv wild type mouse
AU - Rovsing, Louise
AU - Rath, Martin Fredensborg
AU - Møller, Morten
N1 - Copyright © 2013 Wiley Periodicals, Inc., A Wiley Company.
PY - 2013/6/20
Y1 - 2013/6/20
N2 - Vasopressin (AVP) is both a neuroendocrine hormone located in magnocellular neurosecretory neurons of the hypothalamus of mammals but also a neurotransmitter/neuromodulator in the parvocellular suprachiasmatic nucleus (SCN). The SCN is the endogenous clock of the brain and exhibits a prominent circadian AVP-rhythm. We have in this study of the brown 129sv mouse and the visual blind cone-rod homeobox gene knock out mouse (Crx(-/-) ) with degeneration of the retinal rods and cones, but a preserved non-image forming optic system, studied the temporal Avp-expression in both the neurosecretory magnocellular and parvocellular vasopressinergic systems in both genotypes. We here present a detailed mapping of all classical hypothalamo-pituitary and accessory magnocellular nuclei and neurons in the hypothalamus by use of immunohistochemistry and in situ hybridization in both genotypes. Semiquantitative in situ hybridization revealed a very high expression of Avp-mRNA in all the magnocellular nuclei compared to a much lower level in the parvocellular suprachiasmatic nucleus. In a series of mice killed every 4 hours, The Avp-mRNA expression in the SCN showed a significant daily rhythm with a zenith at late day time and nadir during the dark in both the Crx(-/-) and the wild type mouse. None of the magnocellular neurosecretory neurons exhibited a diurnal vasopressin expression. Light stimulation of both genotypes during the dark period did not change the Avp-expression in the SCN. This shows that Avp-expression in the mouse SCN is independent of Crx-regulated photoreceptor systems. J. Comp. Neurol., 2013. © 2013 Wiley Periodicals, Inc.
AB - Vasopressin (AVP) is both a neuroendocrine hormone located in magnocellular neurosecretory neurons of the hypothalamus of mammals but also a neurotransmitter/neuromodulator in the parvocellular suprachiasmatic nucleus (SCN). The SCN is the endogenous clock of the brain and exhibits a prominent circadian AVP-rhythm. We have in this study of the brown 129sv mouse and the visual blind cone-rod homeobox gene knock out mouse (Crx(-/-) ) with degeneration of the retinal rods and cones, but a preserved non-image forming optic system, studied the temporal Avp-expression in both the neurosecretory magnocellular and parvocellular vasopressinergic systems in both genotypes. We here present a detailed mapping of all classical hypothalamo-pituitary and accessory magnocellular nuclei and neurons in the hypothalamus by use of immunohistochemistry and in situ hybridization in both genotypes. Semiquantitative in situ hybridization revealed a very high expression of Avp-mRNA in all the magnocellular nuclei compared to a much lower level in the parvocellular suprachiasmatic nucleus. In a series of mice killed every 4 hours, The Avp-mRNA expression in the SCN showed a significant daily rhythm with a zenith at late day time and nadir during the dark in both the Crx(-/-) and the wild type mouse. None of the magnocellular neurosecretory neurons exhibited a diurnal vasopressin expression. Light stimulation of both genotypes during the dark period did not change the Avp-expression in the SCN. This shows that Avp-expression in the mouse SCN is independent of Crx-regulated photoreceptor systems. J. Comp. Neurol., 2013. © 2013 Wiley Periodicals, Inc.
U2 - 10.1002/cne.23390
DO - 10.1002/cne.23390
M3 - Journal article
C2 - 23784879
VL - 521
SP - 4061
EP - 4074
JO - The Journal of Comparative Neurology
JF - The Journal of Comparative Neurology
SN - 0021-9967
IS - 17
ER -
ID: 47565736