Human leukocyte antigen (HLA) class I restricted epitope discovery in yellow fewer and dengue viruses: importance of HLA binding strength
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Human leukocyte antigen (HLA) class I restricted epitope discovery in yellow fewer and dengue viruses : importance of HLA binding strength. / Lund, Ole; Nascimento, Eduardo J M; Maciel, Milton; Nielsen, Morten; Larsen, Mette Voldby; Lundegaard, Claus; Harndahl, Mikkel; Lamberth, Kasper; Buus, Søren; Salmon, Jérôme; August, Thomas J; Marques, Ernesto T A.
I: P L o S One, Bind 6, Nr. 10, 2011, s. e26494.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Human leukocyte antigen (HLA) class I restricted epitope discovery in yellow fewer and dengue viruses
T2 - importance of HLA binding strength
AU - Lund, Ole
AU - Nascimento, Eduardo J M
AU - Maciel, Milton
AU - Nielsen, Morten
AU - Larsen, Mette Voldby
AU - Lundegaard, Claus
AU - Harndahl, Mikkel
AU - Lamberth, Kasper
AU - Buus, Søren
AU - Salmon, Jérôme
AU - August, Thomas J
AU - Marques, Ernesto T A
PY - 2011
Y1 - 2011
N2 - Epitopes from all available full-length sequences of yellow fever virus (YFV) and dengue fever virus (DENV) restricted by Human Leukocyte Antigen class I (HLA-I) alleles covering 12 HLA-I supertypes were predicted using the NetCTL algorithm. A subset of 179 predicted YFV and 158 predicted DENV epitopes were selected using the EpiSelect algorithm to allow for optimal coverage of viral strains. The selected predicted epitopes were synthesized and approximately 75% were found to bind the predicted restricting HLA molecule with an affinity, K(D), stronger than 500 nM. The immunogenicity of 25 HLA-A*02:01, 28 HLA-A*24:02 and 28 HLA-B*07:02 binding peptides was tested in three HLA-transgenic mice models and led to the identification of 17 HLA-A*02:01, 4 HLA-A*2402 and 4 HLA-B*07:02 immunogenic peptides. The immunogenic peptides bound HLA significantly stronger than the non-immunogenic peptides. All except one of the immunogenic peptides had K(D) below 100 nM and the peptides with K(D) below 5 nM were more likely to be immunogenic. In addition, all the immunogenic peptides that were identified as having a high functional avidity had K(D) below 20 nM. A*02:01 transgenic mice were also inoculated twice with the 17DD YFV vaccine strain. Three of the YFV A*02:01 restricted peptides activated T-cells from the infected mice in vitro. All three peptides that elicited responses had an HLA binding affinity of 2 nM or less. The results indicate the importance of the strength of HLA binding in shaping the immune response.
AB - Epitopes from all available full-length sequences of yellow fever virus (YFV) and dengue fever virus (DENV) restricted by Human Leukocyte Antigen class I (HLA-I) alleles covering 12 HLA-I supertypes were predicted using the NetCTL algorithm. A subset of 179 predicted YFV and 158 predicted DENV epitopes were selected using the EpiSelect algorithm to allow for optimal coverage of viral strains. The selected predicted epitopes were synthesized and approximately 75% were found to bind the predicted restricting HLA molecule with an affinity, K(D), stronger than 500 nM. The immunogenicity of 25 HLA-A*02:01, 28 HLA-A*24:02 and 28 HLA-B*07:02 binding peptides was tested in three HLA-transgenic mice models and led to the identification of 17 HLA-A*02:01, 4 HLA-A*2402 and 4 HLA-B*07:02 immunogenic peptides. The immunogenic peptides bound HLA significantly stronger than the non-immunogenic peptides. All except one of the immunogenic peptides had K(D) below 100 nM and the peptides with K(D) below 5 nM were more likely to be immunogenic. In addition, all the immunogenic peptides that were identified as having a high functional avidity had K(D) below 20 nM. A*02:01 transgenic mice were also inoculated twice with the 17DD YFV vaccine strain. Three of the YFV A*02:01 restricted peptides activated T-cells from the infected mice in vitro. All three peptides that elicited responses had an HLA binding affinity of 2 nM or less. The results indicate the importance of the strength of HLA binding in shaping the immune response.
KW - Amino Acid Sequence
KW - Animals
KW - Dengue Virus
KW - Enzyme-Linked Immunosorbent Assay
KW - Epitopes
KW - Histocompatibility Antigens Class I
KW - Humans
KW - Mice
KW - Mice, Transgenic
KW - Molecular Sequence Data
KW - Yellow Fever Vaccine
KW - Yellow fever virus
U2 - 10.1371/journal.pone.0026494
DO - 10.1371/journal.pone.0026494
M3 - Journal article
C2 - 22039500
VL - 6
SP - e26494
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 10
ER -
ID: 40353962