TY - JOUR

T1 - HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

AU - Olsen, Jørgen

AU - Laustsen, Lotte

AU - Troelsen, J

N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Nucleus; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Hela Cells; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Intestinal Mucosa; Nuclear Proteins; Promoter Regions (Genetics); Protein Binding; Swine; Transcription Factors; Tumor Cells, Cultured

PY - 1994

Y1 - 1994

N2 - The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.

AB - The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.

M3 - Journal article

C2 - 7908650

VL - 342

SP - 325

EP - 328

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 3

ER -