Histamine-forming ability of Lentilactobacillus parabuchneri in reduced salt Cheddar cheese
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Histamine-forming ability of Lentilactobacillus parabuchneri in reduced salt Cheddar cheese. / Møller, Cleide O. de A.; Castro-Mejía, Josué L.; Krych, Lukasz; Rattray, Fergal P.
I: Food Microbiology, Bind 98, 103789, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Histamine-forming ability of Lentilactobacillus parabuchneri in reduced salt Cheddar cheese
AU - Møller, Cleide O. de A.
AU - Castro-Mejía, Josué L.
AU - Krych, Lukasz
AU - Rattray, Fergal P.
PY - 2021
Y1 - 2021
N2 - Lentilactobacillus parabuchneri, a member of the non-starter microbiota in cheese, was recently associated with fast and effective histamine-formation ability, a safety issue. The present study was performed to investigate Lentilactobacillus parabuchneri KUH8, a histamine-producer (HP) in reduced-salt Cheddar cheese. Four cheeses were manufactured: 1) normal-salt (NS); 2) reduced-salt (RS); 3) normal-salt with HP (NS+HP); 4) reduced-salt with HP (RS+HP). Two replicates were produced with milk from the same batch, and the cheeses ripened at 10 and 15 °C. Cheeses were sampled immediately after manufacture and after 1, 3 and 6 months of ripening. Ultra-high-performance-liquid chromatography indicated that with the HP, histamine reached higher levels in reduced-salt cheeses (3.5–3.7% S/M) at 15 °C (86, 1112, 2149 and 3149 mg kg−1), compared to normal-salt cheeses (5.4–6.3% S/M) at 10 °C (78, 584, 593 and 1389 mg kg−1), at each respective cheese-sampling point. Higher salt-content reduced the growth rate of non-starter microbiota, but after six months the levels in all cheeses were similar, according to the ripening temperature: at 10 °C (8.05–8.30 log10 cfu g−1), and at 15 °C (6.00–6.94 log10 cfu g−1). A correlation between increased histamine levels, non-starter-cell development and pH was found. This study highlights the importance of normal-salt content and low-ripening temperature as measures to control histamine-formation and to improve safety in cheese.
AB - Lentilactobacillus parabuchneri, a member of the non-starter microbiota in cheese, was recently associated with fast and effective histamine-formation ability, a safety issue. The present study was performed to investigate Lentilactobacillus parabuchneri KUH8, a histamine-producer (HP) in reduced-salt Cheddar cheese. Four cheeses were manufactured: 1) normal-salt (NS); 2) reduced-salt (RS); 3) normal-salt with HP (NS+HP); 4) reduced-salt with HP (RS+HP). Two replicates were produced with milk from the same batch, and the cheeses ripened at 10 and 15 °C. Cheeses were sampled immediately after manufacture and after 1, 3 and 6 months of ripening. Ultra-high-performance-liquid chromatography indicated that with the HP, histamine reached higher levels in reduced-salt cheeses (3.5–3.7% S/M) at 15 °C (86, 1112, 2149 and 3149 mg kg−1), compared to normal-salt cheeses (5.4–6.3% S/M) at 10 °C (78, 584, 593 and 1389 mg kg−1), at each respective cheese-sampling point. Higher salt-content reduced the growth rate of non-starter microbiota, but after six months the levels in all cheeses were similar, according to the ripening temperature: at 10 °C (8.05–8.30 log10 cfu g−1), and at 15 °C (6.00–6.94 log10 cfu g−1). A correlation between increased histamine levels, non-starter-cell development and pH was found. This study highlights the importance of normal-salt content and low-ripening temperature as measures to control histamine-formation and to improve safety in cheese.
KW - Biogenic amines
KW - Cheese
KW - Lactic acid bacteria
KW - Ripening temperature
KW - Safety
KW - Salt reduction
U2 - 10.1016/j.fm.2021.103789
DO - 10.1016/j.fm.2021.103789
M3 - Journal article
C2 - 33875217
AN - SCOPUS:85103736502
VL - 98
JO - Food Microbiology
JF - Food Microbiology
SN - 0740-0020
M1 - 103789
ER -
ID: 261107374