Histamine immunocytochemistry: a new method for detection of basophils in peripheral blood
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Histamine immunocytochemistry : a new method for detection of basophils in peripheral blood. / Jacobi, H H; Johansson, O; Liang, Y; Nielsen, H V; Thygesen, C; Hansen, J B; Jinquan, T; Skov, P S; Poulsen, Lars K.
I: Journal of Immunological Methods, Bind 237, Nr. 1-2, 03.04.2000, s. 29-37.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Histamine immunocytochemistry
T2 - a new method for detection of basophils in peripheral blood
AU - Jacobi, H H
AU - Johansson, O
AU - Liang, Y
AU - Nielsen, H V
AU - Thygesen, C
AU - Hansen, J B
AU - Jinquan, T
AU - Skov, P S
AU - Poulsen, Lars K.
PY - 2000/4/3
Y1 - 2000/4/3
N2 - We report that basophils in peripheral blood can be stained using histamine immunocytochemistry. The staining is based on the fixation of leucocytes with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (CDI) and the subsequent incubation of these cells with antisera raised against histamine conjugated to different carrier proteins using CDI. The staining appears to be specific for basophils and stained cells can be examined using both fluorescence microscopy and flow cytometry. In addition, histamine immunocytochemistry can be combined with conventional immunocytochemistry by incubating leucocytes with antibodies to cell surface antigens prior to or following fixation of the cells with CDI. Thus, histamine immunocytochemistry may be a valuable tool in future studies of human basophils.
AB - We report that basophils in peripheral blood can be stained using histamine immunocytochemistry. The staining is based on the fixation of leucocytes with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (CDI) and the subsequent incubation of these cells with antisera raised against histamine conjugated to different carrier proteins using CDI. The staining appears to be specific for basophils and stained cells can be examined using both fluorescence microscopy and flow cytometry. In addition, histamine immunocytochemistry can be combined with conventional immunocytochemistry by incubating leucocytes with antibodies to cell surface antigens prior to or following fixation of the cells with CDI. Thus, histamine immunocytochemistry may be a valuable tool in future studies of human basophils.
KW - Alcian Blue
KW - Antibodies, Monoclonal
KW - Antigens, CD
KW - Basophils
KW - Coloring Agents
KW - Ethyldimethylaminopropyl Carbodiimide
KW - Evaluation Studies as Topic
KW - Flow Cytometry
KW - Histamine
KW - Humans
KW - Immunohistochemistry
KW - Leukocytes
KW - Microscopy, Fluorescence
KW - Staining and Labeling
M3 - Journal article
C2 - 10725449
VL - 237
SP - 29
EP - 37
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -
ID: 50846416