High-throughput siRNA screening applied to the ubiquitin-proteasome system
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.
Originalsprog | Engelsk |
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Titel | Proteostasis |
Redaktører | Rune Matthiesen |
Antal sider | 19 |
Forlag | Springer |
Publikationsdato | 2016 |
Sider | 421-439 |
Kapitel | 28 |
ISBN (Trykt) | 978-1-4939-3754-7 |
ISBN (Elektronisk) | 978-1-4939-3756-1 |
DOI | |
Status | Udgivet - 2016 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1449 |
ISSN | 1064-3745 |
ID: 179133797