Group I intron ribozymes

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Group I intron ribozymes. / Nielsen, Henrik.

I: Methods in molecular biology (Clifton, N.J.), Bind 848, 2012, s. 73-89.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nielsen, H 2012, 'Group I intron ribozymes', Methods in molecular biology (Clifton, N.J.), bind 848, s. 73-89. https://doi.org/10.1007/978-1-61779-545-9_6

APA

Nielsen, H. (2012). Group I intron ribozymes. Methods in molecular biology (Clifton, N.J.), 848, 73-89. https://doi.org/10.1007/978-1-61779-545-9_6

Vancouver

Nielsen H. Group I intron ribozymes. Methods in molecular biology (Clifton, N.J.). 2012;848:73-89. https://doi.org/10.1007/978-1-61779-545-9_6

Author

Nielsen, Henrik. / Group I intron ribozymes. I: Methods in molecular biology (Clifton, N.J.). 2012 ; Bind 848. s. 73-89.

Bibtex

@article{b40d696aa42641a796f03a3ce19ecb34,
title = "Group I intron ribozymes",
abstract = "Group I intron ribozymes constitute one of the main classes of ribozymes and have been a particularly important model in the discovery of key concepts in RNA biology as well as in the development of new methods. Compared to other ribozyme classes, group I intron ribozymes display considerable variation both in their structure and the reactions they catalyze. The best described pathway is the splicing pathway that results in a spliced out intron and ligated exons. This is paralleled by the circularization pathway that leads to full-length circular intron and un-ligated exons. In addition, the intronic products of these pathways have the potential to integrate into targets and to form various types of circular RNA molecules. Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols for initial characterization of new group I intron ribozymes.",
keywords = "Biocatalysis, DNA Primers, Hydrolysis, Introns, Nucleic Acid Conformation, RNA Splicing, RNA, Catalytic, Ribonuclease P, Sequence Analysis, RNA, Transcription, Genetic",
author = "Henrik Nielsen",
year = "2012",
doi = "10.1007/978-1-61779-545-9_6",
language = "English",
volume = "848",
pages = "73--89",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Group I intron ribozymes

AU - Nielsen, Henrik

PY - 2012

Y1 - 2012

N2 - Group I intron ribozymes constitute one of the main classes of ribozymes and have been a particularly important model in the discovery of key concepts in RNA biology as well as in the development of new methods. Compared to other ribozyme classes, group I intron ribozymes display considerable variation both in their structure and the reactions they catalyze. The best described pathway is the splicing pathway that results in a spliced out intron and ligated exons. This is paralleled by the circularization pathway that leads to full-length circular intron and un-ligated exons. In addition, the intronic products of these pathways have the potential to integrate into targets and to form various types of circular RNA molecules. Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols for initial characterization of new group I intron ribozymes.

AB - Group I intron ribozymes constitute one of the main classes of ribozymes and have been a particularly important model in the discovery of key concepts in RNA biology as well as in the development of new methods. Compared to other ribozyme classes, group I intron ribozymes display considerable variation both in their structure and the reactions they catalyze. The best described pathway is the splicing pathway that results in a spliced out intron and ligated exons. This is paralleled by the circularization pathway that leads to full-length circular intron and un-ligated exons. In addition, the intronic products of these pathways have the potential to integrate into targets and to form various types of circular RNA molecules. Thus, group I intron ribozymes and associated elements found within group I introns is a rich source of biological phenomena. This chapter provides a strategy and protocols for initial characterization of new group I intron ribozymes.

KW - Biocatalysis

KW - DNA Primers

KW - Hydrolysis

KW - Introns

KW - Nucleic Acid Conformation

KW - RNA Splicing

KW - RNA, Catalytic

KW - Ribonuclease P

KW - Sequence Analysis, RNA

KW - Transcription, Genetic

U2 - 10.1007/978-1-61779-545-9_6

DO - 10.1007/978-1-61779-545-9_6

M3 - Journal article

C2 - 22315064

VL - 848

SP - 73

EP - 89

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 38489890