Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products

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Standard

Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products. / Rasmussen, Henrik Berg; Werge, Thomas.

I: Clinical Chemistry and Laboratory Medicine, Bind 43, Nr. 9, 2005, s. 903-6.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Rasmussen, HB & Werge, T 2005, 'Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products', Clinical Chemistry and Laboratory Medicine, bind 43, nr. 9, s. 903-6. https://doi.org/10.1515/CCLM.2005.154

APA

Rasmussen, H. B., & Werge, T. (2005). Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products. Clinical Chemistry and Laboratory Medicine, 43(9), 903-6. https://doi.org/10.1515/CCLM.2005.154

Vancouver

Rasmussen HB, Werge T. Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products. Clinical Chemistry and Laboratory Medicine. 2005;43(9):903-6. https://doi.org/10.1515/CCLM.2005.154

Author

Rasmussen, Henrik Berg ; Werge, Thomas. / Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products. I: Clinical Chemistry and Laboratory Medicine. 2005 ; Bind 43, Nr. 9. s. 903-6.

Bibtex

@article{5bacca3a3b064a1fbefdf32b339acfbf,
title = "Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products",
abstract = "The 19-bp insertion/deletion polymorphism in the 5' flank of the dopamine beta-hydroxylase (DBH) gene has been associated with psychiatric disorders. We have developed a simple, reliable and inexpensive closed-tube assay for genotyping of this polymorphism based upon T(m) determination of amplified DNA fragments. Mistyping of heterozygote samples due to preferential allele amplification was prevented by use of an optimized concentration of Mg(2+), addition of dimethyl sulfoxide and annealing/extension at an appropriate temperature. Comparison of results achieved by the closed-tube assay and a conventional approach based upon agarose gel electrophoresis of amplified fragments revealed complete concordance between the two procedures. The insights obtained in this study may be utilized to develop assays based upon dissociation analysis of PCR products for genotyping of other insertion/deletion polymorphisms.",
author = "Rasmussen, {Henrik Berg} and Thomas Werge",
year = "2005",
doi = "http://dx.doi.org/10.1515/CCLM.2005.154",
language = "English",
volume = "43",
pages = "903--6",
journal = "Clinical Chemistry and Laboratory Medicine",
issn = "1434-6621",
publisher = "Walterde Gruyter GmbH",
number = "9",

}

RIS

TY - JOUR

T1 - Genotyping of the 19-bp insertion/deletion polymorphism in the 5' flank of beta-hydroxylase gene by dissociation analysis of allele-specific PCR products

AU - Rasmussen, Henrik Berg

AU - Werge, Thomas

PY - 2005

Y1 - 2005

N2 - The 19-bp insertion/deletion polymorphism in the 5' flank of the dopamine beta-hydroxylase (DBH) gene has been associated with psychiatric disorders. We have developed a simple, reliable and inexpensive closed-tube assay for genotyping of this polymorphism based upon T(m) determination of amplified DNA fragments. Mistyping of heterozygote samples due to preferential allele amplification was prevented by use of an optimized concentration of Mg(2+), addition of dimethyl sulfoxide and annealing/extension at an appropriate temperature. Comparison of results achieved by the closed-tube assay and a conventional approach based upon agarose gel electrophoresis of amplified fragments revealed complete concordance between the two procedures. The insights obtained in this study may be utilized to develop assays based upon dissociation analysis of PCR products for genotyping of other insertion/deletion polymorphisms.

AB - The 19-bp insertion/deletion polymorphism in the 5' flank of the dopamine beta-hydroxylase (DBH) gene has been associated with psychiatric disorders. We have developed a simple, reliable and inexpensive closed-tube assay for genotyping of this polymorphism based upon T(m) determination of amplified DNA fragments. Mistyping of heterozygote samples due to preferential allele amplification was prevented by use of an optimized concentration of Mg(2+), addition of dimethyl sulfoxide and annealing/extension at an appropriate temperature. Comparison of results achieved by the closed-tube assay and a conventional approach based upon agarose gel electrophoresis of amplified fragments revealed complete concordance between the two procedures. The insights obtained in this study may be utilized to develop assays based upon dissociation analysis of PCR products for genotyping of other insertion/deletion polymorphisms.

U2 - http://dx.doi.org/10.1515/CCLM.2005.154

DO - http://dx.doi.org/10.1515/CCLM.2005.154

M3 - Journal article

VL - 43

SP - 903

EP - 906

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

SN - 1434-6621

IS - 9

ER -

ID: 48610183