Fluorescence Screening of DNA-AgNCs with Pulsed White Light Excitation
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Fluorescence Screening of DNA-AgNCs with Pulsed White Light Excitation. / Liisberg, Mikkel Baldtzer; Vosch, Tom.
I: Nano Letters, Bind 24, Nr. 26, 2024, s. 7987–7991.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Fluorescence Screening of DNA-AgNCs with Pulsed White Light Excitation
AU - Liisberg, Mikkel Baldtzer
AU - Vosch, Tom
N1 - Funding Information: M.B.L. and T.V. acknowledge funding from the Villum Foundation (VKR023115) and the Independent Research Fund Denmark (0136-00024B). Publisher Copyright: © 2024 The Authors. Published by American Chemical Society.
PY - 2024
Y1 - 2024
N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties dominated by the choice of DNA sequence. Screening methods with ultraviolet excitation and steady state well plate readers have previously been used for deepening the understanding between DNA sequence and emission color of the resulting DNA-AgNCs. Here, we present a new method for screening DNA-AgNCs by using pulsed white light excitation (λex ≈ 490-900 nm). By subtraction and time gating we are able to circumvent the dominating scatter of the white excitation light and extract both temporally and spectrally resolved emission of DNA-AgNCs over the visible to near-infrared range. Additionally, we are able to identify weak long-lived emission, which is often buried underneath the intense nanosecond fluorescence. This new approach will be useful for future screening of DNA-AgNCs (or other novel emissive materials) and aid machine-learning models by providing a richer training data set.
AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties dominated by the choice of DNA sequence. Screening methods with ultraviolet excitation and steady state well plate readers have previously been used for deepening the understanding between DNA sequence and emission color of the resulting DNA-AgNCs. Here, we present a new method for screening DNA-AgNCs by using pulsed white light excitation (λex ≈ 490-900 nm). By subtraction and time gating we are able to circumvent the dominating scatter of the white excitation light and extract both temporally and spectrally resolved emission of DNA-AgNCs over the visible to near-infrared range. Additionally, we are able to identify weak long-lived emission, which is often buried underneath the intense nanosecond fluorescence. This new approach will be useful for future screening of DNA-AgNCs (or other novel emissive materials) and aid machine-learning models by providing a richer training data set.
KW - DNA-AgNCs
KW - Dual Emission
KW - Fluorescence Screening
KW - TCSPC
KW - TWINS
KW - White Light Excitation
U2 - 10.1021/acs.nanolett.4c01561
DO - 10.1021/acs.nanolett.4c01561
M3 - Journal article
C2 - 38905483
AN - SCOPUS:85196907957
VL - 24
SP - 7987
EP - 7991
JO - Nano Letters
JF - Nano Letters
SN - 1530-6984
IS - 26
ER -
ID: 397905913