FBH1 Catalyzes Regression of Stalled Replication Forks
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FBH1 Catalyzes Regression of Stalled Replication Forks. / Fugger, Kasper; Mistrik, Martin; Neelsen, Kai J; Yao, Qi; Zellweger, Ralph; Kousholt, Arne Nedergaard; Haahr, Peter; Chu, Wai Kit; Bartek, Jiri; Lopes, Massimo; Hickson, Ian D; Sørensen, Claus Storgaard.
I: Cell Reports, 10.03.2015.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - FBH1 Catalyzes Regression of Stalled Replication Forks
AU - Fugger, Kasper
AU - Mistrik, Martin
AU - Neelsen, Kai J
AU - Yao, Qi
AU - Zellweger, Ralph
AU - Kousholt, Arne Nedergaard
AU - Haahr, Peter
AU - Chu, Wai Kit
AU - Bartek, Jiri
AU - Lopes, Massimo
AU - Hickson, Ian D
AU - Sørensen, Claus Storgaard
N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2015/3/10
Y1 - 2015/3/10
N2 - DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal. Here, we show that FBH1 catalyzes regression of a model replication fork in vitro and promotes fork regression in vivo in response to replication perturbation. Cells respond to fork stalling by activating checkpoint responses requiring signaling through stress-activated protein kinases. Importantly, we show that FBH1, through its helicase activity, is required for early phosphorylation of ATM substrates such as CHK2 and CtIP as well as hyperphosphorylation of RPA. These phosphorylations occur prior to apparent DNA double-strand break formation. Furthermore, FBH1-dependent signaling promotes checkpoint control and preserves genome integrity. We propose a model whereby FBH1 promotes early checkpoint signaling by remodeling of stalled DNA replication forks.
AB - DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal. Here, we show that FBH1 catalyzes regression of a model replication fork in vitro and promotes fork regression in vivo in response to replication perturbation. Cells respond to fork stalling by activating checkpoint responses requiring signaling through stress-activated protein kinases. Importantly, we show that FBH1, through its helicase activity, is required for early phosphorylation of ATM substrates such as CHK2 and CtIP as well as hyperphosphorylation of RPA. These phosphorylations occur prior to apparent DNA double-strand break formation. Furthermore, FBH1-dependent signaling promotes checkpoint control and preserves genome integrity. We propose a model whereby FBH1 promotes early checkpoint signaling by remodeling of stalled DNA replication forks.
U2 - 10.1016/j.celrep.2015.02.028
DO - 10.1016/j.celrep.2015.02.028
M3 - Journal article
C2 - 25772361
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
ER -
ID: 138140818