Expression of the synaptic vesicle proteins VAMPs/synaptobrevins 1 and 2 in non-neural tissues
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Expression of the synaptic vesicle proteins VAMPs/synaptobrevins 1 and 2 in non-neural tissues. / Ralston, E; Beushausen, S; Ploug, Thorkil.
I: The Journal of Biological Chemistry, Bind 269, Nr. 22, 03.06.1994, s. 15403-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Expression of the synaptic vesicle proteins VAMPs/synaptobrevins 1 and 2 in non-neural tissues
AU - Ralston, E
AU - Beushausen, S
AU - Ploug, Thorkil
PY - 1994/6/3
Y1 - 1994/6/3
N2 - The VAMPs/synaptobrevins (Vp/Sybs) are small integral membrane proteins. Two isoforms, Vp/Syb 1 and Vp/Syb 2, are considered to be specific to neural tissue. They are associated with synaptic vesicles and are believed to play an important role in neurotransmitter release. A third isoform, cellubrevin, has recently been found in non-neural tissues. We now report that the distribution of Vp/Syb 1 and Vp/Syb 2 is wider than previously thought. RNA transcripts for both Vp/Syb 1 and Vp/Syb 2 were found in rat skeletal muscle and in several other rat non-neural tissues, and antibodies specific for Vp/Syb 2 detected a protein in the endoplasmic reticulum-Golgi area of skeletal muscle. Thus Vp/Sybs 1 and 2 are not restricted to the nervous system but appear to be co-expressed with cellubrevin in many different tissues. This redundancy of Vp/Sybs in a single cell may be required to control the specificity of vesicle-target interaction in the several pathways of intracellular vesicle traffic that are operative within each cell.
AB - The VAMPs/synaptobrevins (Vp/Sybs) are small integral membrane proteins. Two isoforms, Vp/Syb 1 and Vp/Syb 2, are considered to be specific to neural tissue. They are associated with synaptic vesicles and are believed to play an important role in neurotransmitter release. A third isoform, cellubrevin, has recently been found in non-neural tissues. We now report that the distribution of Vp/Syb 1 and Vp/Syb 2 is wider than previously thought. RNA transcripts for both Vp/Syb 1 and Vp/Syb 2 were found in rat skeletal muscle and in several other rat non-neural tissues, and antibodies specific for Vp/Syb 2 detected a protein in the endoplasmic reticulum-Golgi area of skeletal muscle. Thus Vp/Sybs 1 and 2 are not restricted to the nervous system but appear to be co-expressed with cellubrevin in many different tissues. This redundancy of Vp/Sybs in a single cell may be required to control the specificity of vesicle-target interaction in the several pathways of intracellular vesicle traffic that are operative within each cell.
KW - Adipose Tissue, Brown
KW - Amino Acid Sequence
KW - Animals
KW - Brain
KW - Endoplasmic Reticulum
KW - Fluorescent Antibody Technique
KW - Gene Expression
KW - Golgi Apparatus
KW - Male
KW - Membrane Proteins
KW - Molecular Sequence Data
KW - Muscles
KW - Nerve Tissue Proteins
KW - Polymerase Chain Reaction
KW - R-SNARE Proteins
KW - Rats
KW - Rats, Wistar
KW - Sequence Homology, Amino Acid
KW - Transcription, Genetic
M3 - Journal article
C2 - 8195178
VL - 269
SP - 15403
EP - 15406
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 22
ER -
ID: 123666177