Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens. / Kucera, Ryan; Bouskila, Joseph; Elkrief, Laurent; Fink-Jensen, Anders; Palmour, Roberta; Bouchard, Jean-François; Ptito, Maurice.
I: Scientific Reports, Bind 8, 8689, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens
AU - Kucera, Ryan
AU - Bouskila, Joseph
AU - Elkrief, Laurent
AU - Fink-Jensen, Anders
AU - Palmour, Roberta
AU - Bouchard, Jean-François
AU - Ptito, Maurice
PY - 2018
Y1 - 2018
N2 - Extensive rodent literature suggests that the endocannabinoid (eCB) system present in the nucleus accumbens (NAc) modulates dopamine (DA) release in this area. However, expression patterns of the cannabinoid receptor type 1 (CB1R), the synthesizing enzyme N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD), and the degradation enzyme fatty acid amide hydrolase (FAAH) in the NAc have not yet been described in non-human primates. The goal of this study is therefore to characterize the expression and localization of the eCB system within the NAc of vervet monkeys (Chlorocebus sabaeus) using Western blots and immunohistochemistry. Results show that CB1R, NAPE-PLD, and FAAH are expressed across the NAc rostrocaudal axis, both in the core and shell. CB1R, NAPE-PLD, and FAAH are localized in medium spiny neurons (MSNs) and fast-spiking GABAergic interneurons (FSIs). Dopaminergic projections and astrocytes did not express CB1R, NAPE-PLD, or FAAH. These data show that the eCB system is present in the vervet monkey NAc and supports its role in the primate brain reward circuit.
AB - Extensive rodent literature suggests that the endocannabinoid (eCB) system present in the nucleus accumbens (NAc) modulates dopamine (DA) release in this area. However, expression patterns of the cannabinoid receptor type 1 (CB1R), the synthesizing enzyme N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD), and the degradation enzyme fatty acid amide hydrolase (FAAH) in the NAc have not yet been described in non-human primates. The goal of this study is therefore to characterize the expression and localization of the eCB system within the NAc of vervet monkeys (Chlorocebus sabaeus) using Western blots and immunohistochemistry. Results show that CB1R, NAPE-PLD, and FAAH are expressed across the NAc rostrocaudal axis, both in the core and shell. CB1R, NAPE-PLD, and FAAH are localized in medium spiny neurons (MSNs) and fast-spiking GABAergic interneurons (FSIs). Dopaminergic projections and astrocytes did not express CB1R, NAPE-PLD, or FAAH. These data show that the eCB system is present in the vervet monkey NAc and supports its role in the primate brain reward circuit.
U2 - 10.1038/s41598-018-26826-2
DO - 10.1038/s41598-018-26826-2
M3 - Journal article
C2 - 29875385
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 8689
ER -
ID: 218086145