Evidence that Transcript Cleavage Is Essential for RNA Polymerase II Transcription and Cell Viability
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Evidence that Transcript Cleavage Is Essential for RNA Polymerase II Transcription and Cell Viability. / Sigurdsson, Stefan; Dirac-Svejstrup, A. Barbara; Svejstrup, Jesper Q.
I: Molecular Cell, Bind 38, Nr. 2, 2010, s. 202-210.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Evidence that Transcript Cleavage Is Essential for RNA Polymerase II Transcription and Cell Viability
AU - Sigurdsson, Stefan
AU - Dirac-Svejstrup, A. Barbara
AU - Svejstrup, Jesper Q.
N1 - Funding Information: This work was supported by an EMBO long-term postdoctoral fellowship to S.S., and by grants from Cancer Research UK, Association of International Cancer Research, and the European Community (Integrated Project DNA repair, LSHG-CT-2005-512113) to J.Q.S. Caroline Kane is thanked for the His-TFIIS expression plasmid; David Jansma and Jim Friesen are thanked for yeast strains; and Dong Wang, Dave Bushnell, Craig Kaplan, and Roger Kornberg are thanked for helpful discussions and advice on RNAPII structure, as well as for comments on the manuscript. Peter Verrijzer and members of the Svejstrup laboratory are thanked for discussions and helpful comments on the manuscript.
PY - 2010
Y1 - 2010
N2 - During transcript elongation in vitro, backtracking of RNA polymerase II (RNAPII) is a frequent occurrence that can lead to transcriptional arrest. The polymerase active site can cleave the transcript during such backtracking, allowing transcription to resume. Transcript cleavage is either stimulated by elongation factor TFIIS or occurs much more slowly in its absence. However, whether backtracking actually occurs in vivo, and whether transcript cleavage is important to escape it, has been unclear. Using a yeast TFIIS mutant that lacks transcript cleavage stimulatory activity and simultaneously inhibits unstimulated cleavage, we now provide evidence that escape from backtracking via transcript cleavage is essential for cell viability and efficient transcript elongation. Our results suggest that transcription problems leading to backtracking are frequent in vivo and that reactivation of backtracked RNAPII is crucial for transcription.
AB - During transcript elongation in vitro, backtracking of RNA polymerase II (RNAPII) is a frequent occurrence that can lead to transcriptional arrest. The polymerase active site can cleave the transcript during such backtracking, allowing transcription to resume. Transcript cleavage is either stimulated by elongation factor TFIIS or occurs much more slowly in its absence. However, whether backtracking actually occurs in vivo, and whether transcript cleavage is important to escape it, has been unclear. Using a yeast TFIIS mutant that lacks transcript cleavage stimulatory activity and simultaneously inhibits unstimulated cleavage, we now provide evidence that escape from backtracking via transcript cleavage is essential for cell viability and efficient transcript elongation. Our results suggest that transcription problems leading to backtracking are frequent in vivo and that reactivation of backtracked RNAPII is crucial for transcription.
KW - DNA
KW - RNA
U2 - 10.1016/j.molcel.2010.02.026
DO - 10.1016/j.molcel.2010.02.026
M3 - Journal article
C2 - 20417599
AN - SCOPUS:77950998789
VL - 38
SP - 202
EP - 210
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 2
ER -
ID: 330899657