Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function
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Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function. / Albrethsen, Jakob; Johannsen, Trine Holm; Jørgensen, Niels; Frederiksen, Hanne; Sennels, Henriette P.; Jorgensen, Henrik Loevendahl; Fahrenkrug, Jan; Petersen, Jorgen Holm; Linneberg, Allan; Nordkap, Loa; Bang, Anne Kirstine; Andersson, Anna-Maria; Juul, Anders.
I: Journal of Clinical Endocrinology & Metabolism, Bind 105, Nr. 6, 2020, s. 1868-1877.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function
AU - Albrethsen, Jakob
AU - Johannsen, Trine Holm
AU - Jørgensen, Niels
AU - Frederiksen, Hanne
AU - Sennels, Henriette P.
AU - Jorgensen, Henrik Loevendahl
AU - Fahrenkrug, Jan
AU - Petersen, Jorgen Holm
AU - Linneberg, Allan
AU - Nordkap, Loa
AU - Bang, Anne Kirstine
AU - Andersson, Anna-Maria
AU - Juul, Anders
PY - 2020
Y1 - 2020
N2 - Background: The peptide hormone insulin-like factor 3 (INSL3) is a marker for Leydig cell function and the clinical use of serum INSL3 measurements has been suggested by several groups.Aim: (1) To establish a reference range for liquid chromatography-tandem mass spectrometry (LC-MS/MS) of serum INSL3 in healthy boys and men; and (2) to compare the associations of serum INSL3 and testosterone (T) to pubertal stage, lifestyle factors, diurnal variation, body composition, and human chorionic gonadotropin (hCG) stimulation.Results: In a reference range based on LC-MS/MS analysis of serum from 1073 boys and men, INSL3 increased from levels close to the detection limit (0.03 mu g/L) in prepubertal boys to a maximum mean level of 1.3 mu g/L (95% CI, 0.9-2.7) in young men (19-40 years of age) and decreased slightly in older men (0.1 mu g/L per decade). Serum T, but not INSL3, was associated with body mass index or body fat percentage and with alcohol consumption. Smoking was positively associated with serum T, but negatively associated with INSL3. There were significant diurnal variations in both INSL3 and T in men (P <0.001), but serum INSL3 varied substantially less, compared with serum T (+/- 11% vs +/- 26%). Mean serum INSL3 increased after hCG stimulation, but less than T (+ 17% vs +/- 53%), In both healthy men and in patients suspected of testicular failure, baseline serum INSL3 was more closely associated to the hCG-induced increase in serum T than baseline T itself.Conclusion: Measurement of serum INSL3 by LC-MS/MS has promise as a marker of testicular disorders.
AB - Background: The peptide hormone insulin-like factor 3 (INSL3) is a marker for Leydig cell function and the clinical use of serum INSL3 measurements has been suggested by several groups.Aim: (1) To establish a reference range for liquid chromatography-tandem mass spectrometry (LC-MS/MS) of serum INSL3 in healthy boys and men; and (2) to compare the associations of serum INSL3 and testosterone (T) to pubertal stage, lifestyle factors, diurnal variation, body composition, and human chorionic gonadotropin (hCG) stimulation.Results: In a reference range based on LC-MS/MS analysis of serum from 1073 boys and men, INSL3 increased from levels close to the detection limit (0.03 mu g/L) in prepubertal boys to a maximum mean level of 1.3 mu g/L (95% CI, 0.9-2.7) in young men (19-40 years of age) and decreased slightly in older men (0.1 mu g/L per decade). Serum T, but not INSL3, was associated with body mass index or body fat percentage and with alcohol consumption. Smoking was positively associated with serum T, but negatively associated with INSL3. There were significant diurnal variations in both INSL3 and T in men (P <0.001), but serum INSL3 varied substantially less, compared with serum T (+/- 11% vs +/- 26%). Mean serum INSL3 increased after hCG stimulation, but less than T (+ 17% vs +/- 53%), In both healthy men and in patients suspected of testicular failure, baseline serum INSL3 was more closely associated to the hCG-induced increase in serum T than baseline T itself.Conclusion: Measurement of serum INSL3 by LC-MS/MS has promise as a marker of testicular disorders.
KW - INSL3
KW - testosterone
KW - normal range
KW - diurnal variation
KW - Leydig cell
KW - LC-MS/MS
KW - LIFE-STYLE FACTORS
KW - HEALTHY BOYS
KW - TESTOSTERONE
KW - MEN
KW - AGE
KW - ASSOCIATIONS
KW - DECLINE
KW - HORMONE
U2 - 10.1210/clinem/dgaa145
DO - 10.1210/clinem/dgaa145
M3 - Journal article
C2 - 32211773
VL - 105
SP - 1868
EP - 1877
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 6
ER -
ID: 246779212