Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

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Standard

Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate. / Eirheim, Heidi Ugelstad; Bundgaard, Christoffer; Nielsen, Hanne Mørck.

I: Toxicology in Vitro, Bind 18, Nr. 5, 2004, s. 649-57.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Eirheim, HU, Bundgaard, C & Nielsen, HM 2004, 'Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate', Toxicology in Vitro, bind 18, nr. 5, s. 649-57. https://doi.org/10.1016/j.tiv.2004.02.003

APA

Eirheim, H. U., Bundgaard, C., & Nielsen, H. M. (2004). Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate. Toxicology in Vitro, 18(5), 649-57. https://doi.org/10.1016/j.tiv.2004.02.003

Vancouver

Eirheim HU, Bundgaard C, Nielsen HM. Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate. Toxicology in Vitro. 2004;18(5):649-57. https://doi.org/10.1016/j.tiv.2004.02.003

Author

Eirheim, Heidi Ugelstad ; Bundgaard, Christoffer ; Nielsen, Hanne Mørck. / Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate. I: Toxicology in Vitro. 2004 ; Bind 18, Nr. 5. s. 649-57.

Bibtex

@article{159af813001e4c38a895a3cae36acf3e,
title = "Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate",
abstract = "The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P ",
keywords = "Adenosine Triphosphate, Cell Line, Tumor, Cell Membrane Permeability, Cell Proliferation, Detergents, Dose-Response Relationship, Drug, Epithelial Cells, Glycocholic Acid, Humans, Inhibitory Concentration 50, Mannitol, Methylphenazonium Methosulfate, Mouth Mucosa, Neutral Red, Proteins, Reproducibility of Results, Tetrazolium Salts, Thiazoles, Toxicity Tests",
author = "Eirheim, {Heidi Ugelstad} and Christoffer Bundgaard and Nielsen, {Hanne M{\o}rck}",
year = "2004",
doi = "10.1016/j.tiv.2004.02.003",
language = "English",
volume = "18",
pages = "649--57",
journal = "Toxicology in Vitro",
issn = "0887-2333",
publisher = "Pergamon Press",
number = "5",

}

RIS

TY - JOUR

T1 - Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

AU - Eirheim, Heidi Ugelstad

AU - Bundgaard, Christoffer

AU - Nielsen, Hanne Mørck

PY - 2004

Y1 - 2004

N2 - The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P

AB - The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P

KW - Adenosine Triphosphate

KW - Cell Line, Tumor

KW - Cell Membrane Permeability

KW - Cell Proliferation

KW - Detergents

KW - Dose-Response Relationship, Drug

KW - Epithelial Cells

KW - Glycocholic Acid

KW - Humans

KW - Inhibitory Concentration 50

KW - Mannitol

KW - Methylphenazonium Methosulfate

KW - Mouth Mucosa

KW - Neutral Red

KW - Proteins

KW - Reproducibility of Results

KW - Tetrazolium Salts

KW - Thiazoles

KW - Toxicity Tests

U2 - 10.1016/j.tiv.2004.02.003

DO - 10.1016/j.tiv.2004.02.003

M3 - Journal article

C2 - 15251183

VL - 18

SP - 649

EP - 657

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

IS - 5

ER -

ID: 43890885