Evaluation, including effects of storage and repeated freezing and thawing, of a method for measurement of urinary creatinine
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Evaluation, including effects of storage and repeated freezing and thawing, of a method for measurement of urinary creatinine. / Garde, A H; Hansen, Åse Marie; Kristiansen, J.
I: Scandinavian Journal of Clinical & Laboratory Investigation, Bind 63, Nr. 7-8, 2003, s. 521-4.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Evaluation, including effects of storage and repeated freezing and thawing, of a method for measurement of urinary creatinine
AU - Garde, A H
AU - Hansen, Åse Marie
AU - Kristiansen, J
PY - 2003
Y1 - 2003
N2 - The aims of this study were to elucidate to what extent storage and repeated freezing and thawing influenced the concentration of creatinine in urine samples and to evaluate the method for determination of creatinine in urine. The creatinine method was based on the well-known Jaffe's reaction and measured on a COBAS Mira autoanalyser from Roche. The main findings were that samples for analysis of creatinine should be kept at a temperature of -20 degrees C or lower and frozen and thawed only once. The limit of detection, determined as 3 x SD of 20 determinations of a sample at a low concentration (6.1 mmol/L), was 0.3 mmol/L, and the recovery of a certified reference material was 97%. The relative precision at 3.15 mmol/L was 2.3%. It was concluded that the method is appropriate for measurement of urinary creatinine.
AB - The aims of this study were to elucidate to what extent storage and repeated freezing and thawing influenced the concentration of creatinine in urine samples and to evaluate the method for determination of creatinine in urine. The creatinine method was based on the well-known Jaffe's reaction and measured on a COBAS Mira autoanalyser from Roche. The main findings were that samples for analysis of creatinine should be kept at a temperature of -20 degrees C or lower and frozen and thawed only once. The limit of detection, determined as 3 x SD of 20 determinations of a sample at a low concentration (6.1 mmol/L), was 0.3 mmol/L, and the recovery of a certified reference material was 97%. The relative precision at 3.15 mmol/L was 2.3%. It was concluded that the method is appropriate for measurement of urinary creatinine.
KW - Clinical Laboratory Techniques
KW - Cold Temperature
KW - Creatinine
KW - Data Interpretation, Statistical
KW - Freezing
KW - Humans
KW - Reference Standards
KW - Regression Analysis
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Temperature
M3 - Journal article
C2 - 14743962
VL - 63
SP - 521
EP - 524
JO - Scandinavian Journal of Clinical & Laboratory Investigation
JF - Scandinavian Journal of Clinical & Laboratory Investigation
SN - 0036-5513
IS - 7-8
ER -
ID: 48865387