Essentials in the acquisition, interpretation, and reporting of plant metabolite profiles
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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Essentials in the acquisition, interpretation, and reporting of plant metabolite profiles. / Çiçek, Serhat S.; Mangoni, Alfonso; Hanschen, Franziska S.; Agerbirk, Niels; Zidorn, Christian.
I: Phytochemistry, Bind 220, 114004, 2024.Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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TY - JOUR
T1 - Essentials in the acquisition, interpretation, and reporting of plant metabolite profiles
AU - Çiçek, Serhat S.
AU - Mangoni, Alfonso
AU - Hanschen, Franziska S.
AU - Agerbirk, Niels
AU - Zidorn, Christian
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024
Y1 - 2024
N2 - Plant metabolite profiling reveals the diversity of secondary or specialized metabolites in the plant kingdom with its hundreds of thousands of species. Specialized plant metabolites constitute a vast class of chemicals posing significant challenges in analytical chemistry. In order to be of maximum scientific relevance, reports dealing with these compounds and their source species must be transparent, make use of standards and reference materials, and be based on correctly and traceably identified plant material. Essential aspects in qualitative plant metabolite profiling include: (i) critical review of previous literature and a reasoned sampling strategy; (ii) transparent plant sampling with wild material documented by vouchers in public herbaria and, optimally, seed banks; (iii) if possible, inclusion of generally available reference plant material; (iv) transparent, documented state-of-the art chemical analysis, ideally including chemical reference standards; (v) testing for artefacts during preparative extraction and isolation, using gentle analytical methods; (vi) careful chemical data interpretation, avoiding over- and misinterpretation and taking into account phytochemical complexity when assigning identification confidence levels, and (vii) taking all previous scientific knowledge into account in reporting the scientific data. From the current stage of the phytochemical literature, selected comments and suggestions are given. In the past, proposed revisions of botanical taxonomy were sometimes based on metabolite profiles, but this approach (“chemosystematics” or “chemotaxonomy”) is outdated due to the advent of DNA sequence-based phylogenies. In contrast, systematic comparisons of plant metabolite profiles in a known phylogenetic framework remain relevant. This approach, known as chemophenetics, allows characterizing species and clades based on their array of specialized metabolites, aids in deducing the evolution of biosynthetic pathways and coevolution, and can serve in identifying new sources of rare and economically interesting natural products.
AB - Plant metabolite profiling reveals the diversity of secondary or specialized metabolites in the plant kingdom with its hundreds of thousands of species. Specialized plant metabolites constitute a vast class of chemicals posing significant challenges in analytical chemistry. In order to be of maximum scientific relevance, reports dealing with these compounds and their source species must be transparent, make use of standards and reference materials, and be based on correctly and traceably identified plant material. Essential aspects in qualitative plant metabolite profiling include: (i) critical review of previous literature and a reasoned sampling strategy; (ii) transparent plant sampling with wild material documented by vouchers in public herbaria and, optimally, seed banks; (iii) if possible, inclusion of generally available reference plant material; (iv) transparent, documented state-of-the art chemical analysis, ideally including chemical reference standards; (v) testing for artefacts during preparative extraction and isolation, using gentle analytical methods; (vi) careful chemical data interpretation, avoiding over- and misinterpretation and taking into account phytochemical complexity when assigning identification confidence levels, and (vii) taking all previous scientific knowledge into account in reporting the scientific data. From the current stage of the phytochemical literature, selected comments and suggestions are given. In the past, proposed revisions of botanical taxonomy were sometimes based on metabolite profiles, but this approach (“chemosystematics” or “chemotaxonomy”) is outdated due to the advent of DNA sequence-based phylogenies. In contrast, systematic comparisons of plant metabolite profiles in a known phylogenetic framework remain relevant. This approach, known as chemophenetics, allows characterizing species and clades based on their array of specialized metabolites, aids in deducing the evolution of biosynthetic pathways and coevolution, and can serve in identifying new sources of rare and economically interesting natural products.
KW - Chemophenetics
KW - Chemosystematics
KW - Chemotaxonomy
KW - Confidence levels
KW - Interpretation
KW - Mass spectrometry
KW - Misidentification
KW - NMR
KW - Plant metabolite profiling
KW - References
KW - Standards
KW - Transparency
U2 - 10.1016/j.phytochem.2024.114004
DO - 10.1016/j.phytochem.2024.114004
M3 - Review
C2 - 38331135
AN - SCOPUS:85185282582
VL - 220
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
M1 - 114004
ER -
ID: 387741865