Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases: Targeted DNA Methylation Analysis Methods

Publikation: Bog/antologi/afhandling/rapport › Bog › Formidling

Standard

Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases : Targeted DNA Methylation Analysis Methods. / Cheishvili, D.; Petropoulos, S.; Christiansen, S.; Szyf, M.

Humana Press, New York, NY : Humana Press, 2017. (Methods in Pharmacology and Toxicology).

Publikation: Bog/antologi/afhandling/rapport › Bog › Formidling

Harvard

Cheishvili, D, Petropoulos, S, Christiansen, S & Szyf, M 2017, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases: Targeted DNA Methylation Analysis Methods. Methods in Pharmacology and Toxicology, Humana Press, Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6743-8_3

APA

Cheishvili, D., Petropoulos, S., Christiansen, S., & Szyf, M. (2017). Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases: Targeted DNA Methylation Analysis Methods. Humana Press. Methods in Pharmacology and Toxicology https://doi.org/10.1007/978-1-4939-6743-8_3

Vancouver

Cheishvili D, Petropoulos S, Christiansen S, Szyf M. Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases: Targeted DNA Methylation Analysis Methods. Humana Press, New York, NY: Humana Press, 2017. (Methods in Pharmacology and Toxicology). https://doi.org/10.1007/978-1-4939-6743-8_3

Author

Cheishvili, D. ; Petropoulos, S. ; Christiansen, S. ; Szyf, M. / Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases : Targeted DNA Methylation Analysis Methods. Humana Press, New York, NY : Humana Press, 2017. (Methods in Pharmacology and Toxicology).

Bibtex

@book{2139e307df3241268f9b63d1550e0ebf,
title = "Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases: Targeted DNA Methylation Analysis Methods",
abstract = "DNA methylation is an important enzymatic covalent modification of DNA that plays an important role in genome regulation. DNA methylation patterns are fashioned during development and could be altered in response to experience and exposure. Aberrations in DNA methylation patterns are noted in cancer and other diseases. It is therefore extremely important to accurately quantify DNA methylation states for studying physiology and disease as well as for using DNA methylation markers in diagnosis. Here, we review the most commonly used methods for quantifying DNA methylation states of single genes: Pyrosequencing, Quantitative Methylated DNA Immunoprecipitation (qMeDIP), and methylation-sensitive high resolution melting (MS-HRM). Each method is described and required steps are detailed. We also discuss the advantages and disadvantages of the different methods.",
keywords = "[DNA methylation, Methylation-sensitive high resol",
author = "D. Cheishvili and S. Petropoulos and S. Christiansen and M. Szyf",
year = "2017",
doi = "10.1007/978-1-4939-6743-8_3",
language = "English",
isbn = "978-1-4939-6741-4",
series = "Methods in Pharmacology and Toxicology",
publisher = "Humana Press",
address = "United States",

}

RIS

TY - BOOK

T1 - Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

T2 - Targeted DNA Methylation Analysis Methods

AU - Cheishvili, D.

AU - Petropoulos, S.

AU - Christiansen, S.

AU - Szyf, M.

PY - 2017

Y1 - 2017

N2 - DNA methylation is an important enzymatic covalent modification of DNA that plays an important role in genome regulation. DNA methylation patterns are fashioned during development and could be altered in response to experience and exposure. Aberrations in DNA methylation patterns are noted in cancer and other diseases. It is therefore extremely important to accurately quantify DNA methylation states for studying physiology and disease as well as for using DNA methylation markers in diagnosis. Here, we review the most commonly used methods for quantifying DNA methylation states of single genes: Pyrosequencing, Quantitative Methylated DNA Immunoprecipitation (qMeDIP), and methylation-sensitive high resolution melting (MS-HRM). Each method is described and required steps are detailed. We also discuss the advantages and disadvantages of the different methods.

AB - DNA methylation is an important enzymatic covalent modification of DNA that plays an important role in genome regulation. DNA methylation patterns are fashioned during development and could be altered in response to experience and exposure. Aberrations in DNA methylation patterns are noted in cancer and other diseases. It is therefore extremely important to accurately quantify DNA methylation states for studying physiology and disease as well as for using DNA methylation markers in diagnosis. Here, we review the most commonly used methods for quantifying DNA methylation states of single genes: Pyrosequencing, Quantitative Methylated DNA Immunoprecipitation (qMeDIP), and methylation-sensitive high resolution melting (MS-HRM). Each method is described and required steps are detailed. We also discuss the advantages and disadvantages of the different methods.

KW - [DNA methylation, Methylation-sensitive high resol

U2 - 10.1007/978-1-4939-6743-8_3

DO - 10.1007/978-1-4939-6743-8_3

M3 - Book

SN - 978-1-4939-6741-4

T3 - Methods in Pharmacology and Toxicology

BT - Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

PB - Humana Press

CY - Humana Press, New York, NY

ER -

ID: 182892028