Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results
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In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2–30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.
Originalsprog | Engelsk |
---|---|
Tidsskrift | APMIS |
Vol/bind | 130 |
Udgave nummer | 10 |
Sider (fra-til) | 612-617 |
Antal sider | 6 |
ISSN | 0903-4641 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
The laboratory staff at the Department of Clinical Microbiology Hvidovre Hospital and Rigshospitalet are thanked for the extra work due to this investigation.
Publisher Copyright:
© 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.
ID: 320505134