Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results

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Standard

Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene : be alert of false-negative test results. / Jørgensen, Rikke Lind; Lerche, Christian Johann; Pedersen, Martin Schou; Kirkby, Nikolai Soren; Botnen, Amanda Bolt; Trebbien, Ramona; Nilsson-Møller, Stephen; Pinholt, Mette; Nielsen, Alex Christian Yde; Westh, Henrik; Lisby, Jan Gorm; Schneider, Uffe Vest.

I: APMIS, Bind 130, Nr. 10, 2022, s. 612-617.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Jørgensen, RL, Lerche, CJ, Pedersen, MS, Kirkby, NS, Botnen, AB, Trebbien, R, Nilsson-Møller, S, Pinholt, M, Nielsen, ACY, Westh, H, Lisby, JG & Schneider, UV 2022, 'Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results', APMIS, bind 130, nr. 10, s. 612-617. https://doi.org/10.1111/apm.13262

APA

Jørgensen, R. L., Lerche, C. J., Pedersen, M. S., Kirkby, N. S., Botnen, A. B., Trebbien, R., Nilsson-Møller, S., Pinholt, M., Nielsen, A. C. Y., Westh, H., Lisby, J. G., & Schneider, U. V. (2022). Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results. APMIS, 130(10), 612-617. https://doi.org/10.1111/apm.13262

Vancouver

Jørgensen RL, Lerche CJ, Pedersen MS, Kirkby NS, Botnen AB, Trebbien R o.a. Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results. APMIS. 2022;130(10):612-617. https://doi.org/10.1111/apm.13262

Author

Jørgensen, Rikke Lind ; Lerche, Christian Johann ; Pedersen, Martin Schou ; Kirkby, Nikolai Soren ; Botnen, Amanda Bolt ; Trebbien, Ramona ; Nilsson-Møller, Stephen ; Pinholt, Mette ; Nielsen, Alex Christian Yde ; Westh, Henrik ; Lisby, Jan Gorm ; Schneider, Uffe Vest. / Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene : be alert of false-negative test results. I: APMIS. 2022 ; Bind 130, Nr. 10. s. 612-617.

Bibtex

@article{5b26908599674b378b0ba550a71f6540,
title = "Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results",
abstract = "In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2–30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.",
keywords = "Assay, diagnostic, genetic drift, H3N2, M gene, mutations, RT-PCR, sequencing, surveillance",
author = "J{\o}rgensen, {Rikke Lind} and Lerche, {Christian Johann} and Pedersen, {Martin Schou} and Kirkby, {Nikolai Soren} and Botnen, {Amanda Bolt} and Ramona Trebbien and Stephen Nilsson-M{\o}ller and Mette Pinholt and Nielsen, {Alex Christian Yde} and Henrik Westh and Lisby, {Jan Gorm} and Schneider, {Uffe Vest}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.",
year = "2022",
doi = "10.1111/apm.13262",
language = "English",
volume = "130",
pages = "612--617",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "10",

}

RIS

TY - JOUR

T1 - Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene

T2 - be alert of false-negative test results

AU - Jørgensen, Rikke Lind

AU - Lerche, Christian Johann

AU - Pedersen, Martin Schou

AU - Kirkby, Nikolai Soren

AU - Botnen, Amanda Bolt

AU - Trebbien, Ramona

AU - Nilsson-Møller, Stephen

AU - Pinholt, Mette

AU - Nielsen, Alex Christian Yde

AU - Westh, Henrik

AU - Lisby, Jan Gorm

AU - Schneider, Uffe Vest

N1 - Publisher Copyright: © 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.

PY - 2022

Y1 - 2022

N2 - In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2–30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.

AB - In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2–30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.

KW - Assay

KW - diagnostic

KW - genetic drift

KW - H3N2

KW - M gene

KW - mutations

KW - RT-PCR

KW - sequencing

KW - surveillance

U2 - 10.1111/apm.13262

DO - 10.1111/apm.13262

M3 - Journal article

C2 - 35836366

AN - SCOPUS:85135608051

VL - 130

SP - 612

EP - 617

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 10

ER -

ID: 320505134