Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes
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Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes. / Stallknecht, B; Andersen, P H; Vinten, J; Bendtsen, L L; Sibbersen, J; Pedersen, O; Galbo, H.
I: American Journal of Physiology: Endocrinology and Metabolism, Bind 265, Nr. 1 Pt 1, 1993, s. E128-E134.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes
AU - Stallknecht, B
AU - Andersen, P H
AU - Vinten, J
AU - Bendtsen, L L
AU - Sibbersen, J
AU - Pedersen, O
AU - Galbo, H
N1 - Keywords: 3-O-Methylglucose; Adipose Tissue; Animals; Biological Transport; Glucose Transporter Type 1; Glucose Transporter Type 4; Male; Methylglucosides; Monosaccharide Transport Proteins; Muscle Proteins; Osmolar Concentration; Physical Conditioning, Animal; RNA, Messenger; Rats; Rats, Wistar
PY - 1993
Y1 - 1993
N2 - Physical training increases insulin-stimulated glucose transport and the number of glucose transporters in adipocytes measured by cytochalasin B binding. In the present study we used immunoblotting to measure the abundance of two glucose transporters (GLUT-4, GLUT-1) in white adipocytes from trained rats. Furthermore, the abundance of the mRNAs for these proteins and glucose transport was measured. Rats were swim-trained for 10 wk, and adipocytes were isolated from epididymal fat pads. The amount of GLUT-4/adipocyte volume unit was significantly higher in trained animals compared with both age- and cell size-matched animals. The amount of GLUT-4 mRNA was also increased by training and it decreased with increasing age. Furthermore, young age as well as training was accompanied by relatively low GLUT-4 protein/mRNA and relatively high overall GLUT-4 efficiency (recruitability and/or intrinsic activity). GLUT-1 protein and mRNA levels/adipocyte volume did not change with age or training.
AB - Physical training increases insulin-stimulated glucose transport and the number of glucose transporters in adipocytes measured by cytochalasin B binding. In the present study we used immunoblotting to measure the abundance of two glucose transporters (GLUT-4, GLUT-1) in white adipocytes from trained rats. Furthermore, the abundance of the mRNAs for these proteins and glucose transport was measured. Rats were swim-trained for 10 wk, and adipocytes were isolated from epididymal fat pads. The amount of GLUT-4/adipocyte volume unit was significantly higher in trained animals compared with both age- and cell size-matched animals. The amount of GLUT-4 mRNA was also increased by training and it decreased with increasing age. Furthermore, young age as well as training was accompanied by relatively low GLUT-4 protein/mRNA and relatively high overall GLUT-4 efficiency (recruitability and/or intrinsic activity). GLUT-1 protein and mRNA levels/adipocyte volume did not change with age or training.
M3 - Journal article
C2 - 8338145
VL - 265
SP - E128-E134
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1 Pt 1
ER -
ID: 271106