Eccentric contractions affect muscle membrane phospholipid fatty acid composition in rats
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Eccentric contractions affect muscle membrane phospholipid fatty acid composition in rats. / Helge, Jørn Wulff; Therkildsen, K J; Jørgensen, T B; Wu, B J; Storlien, L H; Asp, Stefan Erik.
I: Experimental Physiology, Bind 86, Nr. 5, 01.09.2001, s. 599-604.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Eccentric contractions affect muscle membrane phospholipid fatty acid composition in rats
AU - Helge, Jørn Wulff
AU - Therkildsen, K J
AU - Jørgensen, T B
AU - Wu, B J
AU - Storlien, L H
AU - Asp, Stefan Erik
PY - 2001/9/1
Y1 - 2001/9/1
N2 - This study investigated if prior eccentric contractions, and thus mechanical strain and muscle damage, exert an effect on the muscle membrane phospholipid fatty acid composition in rats, and whether a possible effect could be attenuated by dietary supplements. Twenty-three rats were randomised to three groups who received chow with added fish oil (n = 8), vitamin C (n = 8) or no supplement (n = 7). After 3 weeks of feeding, calf muscles on one side were stimulated electrically during anaesthesia causing eccentric contractions. Two days later the white gastrocnemius, a part of the stimulated calf muscle, was excised from both legs. In the muscles stimulated to contract eccentrically, compared to the control muscles, the proportion of arachidonic acid, C20:4,n-6 (17.7 +/- 0.6; 16.4 +/- 0.4% of total fatty acids, respectively) and docosapentanoeic acid, C22:5,n-3 (2.9 +/- 0.1 and 2.7 +/- 0.1% of total fatty acids, respectively) was uniformly higher across groups (P <0.02) with no differences between diet groups. The proportion of long chain polyunsaturates was also significantly higher in the eccentrically contracted (39.9 +/- 0.6% of total fatty acids) compared to the control leg (38.2 +/- 0.6% of total fatty acids; P <0.01). In contrast no differences were observed in the fatty acid composition of the triacylglycerols stored within the muscle. Thus one severe bout of eccentric contractions modulates the fatty acid composition of the muscle membrane phospholipids when compared to a control leg, and supplemental intake of fish oil or vitamin C did not attenuate this effect.
AB - This study investigated if prior eccentric contractions, and thus mechanical strain and muscle damage, exert an effect on the muscle membrane phospholipid fatty acid composition in rats, and whether a possible effect could be attenuated by dietary supplements. Twenty-three rats were randomised to three groups who received chow with added fish oil (n = 8), vitamin C (n = 8) or no supplement (n = 7). After 3 weeks of feeding, calf muscles on one side were stimulated electrically during anaesthesia causing eccentric contractions. Two days later the white gastrocnemius, a part of the stimulated calf muscle, was excised from both legs. In the muscles stimulated to contract eccentrically, compared to the control muscles, the proportion of arachidonic acid, C20:4,n-6 (17.7 +/- 0.6; 16.4 +/- 0.4% of total fatty acids, respectively) and docosapentanoeic acid, C22:5,n-3 (2.9 +/- 0.1 and 2.7 +/- 0.1% of total fatty acids, respectively) was uniformly higher across groups (P <0.02) with no differences between diet groups. The proportion of long chain polyunsaturates was also significantly higher in the eccentrically contracted (39.9 +/- 0.6% of total fatty acids) compared to the control leg (38.2 +/- 0.6% of total fatty acids; P <0.01). In contrast no differences were observed in the fatty acid composition of the triacylglycerols stored within the muscle. Thus one severe bout of eccentric contractions modulates the fatty acid composition of the muscle membrane phospholipids when compared to a control leg, and supplemental intake of fish oil or vitamin C did not attenuate this effect.
KW - Animals
KW - Antioxidants
KW - Ascorbic Acid
KW - Blood Glucose
KW - Dietary Fats
KW - Electric Stimulation
KW - Fatty Acids
KW - Fish Oils
KW - Lactic Acid
KW - Male
KW - Muscle Contraction
KW - Muscle, Skeletal
KW - Phospholipids
KW - Random Allocation
KW - Rats
KW - Rats, Wistar
KW - Stress, Mechanical
KW - Triglycerides
M3 - Journal article
C2 - 11571487
VL - 86
SP - 599
EP - 604
JO - Experimental Physiology
JF - Experimental Physiology
SN - 0958-0670
IS - 5
ER -
ID: 33862302