EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
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EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification. / Virreira Winter, Sebastian; Meier, Florian; Wichmann, Christoph; Cox, Juergen; Mann, Matthias; Meissner, Felix.
I: Nature Methods, Bind 15, Nr. 7, 2018, s. 527-530.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
AU - Virreira Winter, Sebastian
AU - Meier, Florian
AU - Wichmann, Christoph
AU - Cox, Juergen
AU - Mann, Matthias
AU - Meissner, Felix
PY - 2018
Y1 - 2018
N2 - We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.
AB - We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.
U2 - 10.1038/s41592-018-0037-8
DO - 10.1038/s41592-018-0037-8
M3 - Journal article
C2 - 29915187
VL - 15
SP - 527
EP - 530
JO - Nature Methods
JF - Nature Methods
SN - 1548-7091
IS - 7
ER -
ID: 198266076