Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells
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Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells. / Osteil, Pierre; Studdert, Josh B.; Goh, Hwee Ngee; Wilkie, Emilie E.; Fan, Xiaochen; Khoo, Poh Lynn; Peng, Guangdun; Salehin, Nazmus; Knowles, Hilary; Han, Jing Dong J.; Jing, Naihe; Fossat, Nicolas; Tam, Patrick P.L.
I: Development (Cambridge), Bind 146, Nr. 7, dev172858, 2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells
AU - Osteil, Pierre
AU - Studdert, Josh B.
AU - Goh, Hwee Ngee
AU - Wilkie, Emilie E.
AU - Fan, Xiaochen
AU - Khoo, Poh Lynn
AU - Peng, Guangdun
AU - Salehin, Nazmus
AU - Knowles, Hilary
AU - Han, Jing Dong J.
AU - Jing, Naihe
AU - Fossat, Nicolas
AU - Tam, Patrick P.L.
PY - 2019
Y1 - 2019
N2 - During embryogenesis, the stringent regulation of Wnt activity is crucial for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore be crucial for the allocation of epiblast cells to ectoderm progenitors during gastrulation. To test this hypothesis, we examined the lineage characteristics of epiblast stem cells (EpiSCs) that were derived and maintained under different signalling conditions. We showed that suppression of Wnt activity enhanced the ectoderm propensity of the EpiSCs. Neuroectoderm differentiation of these EpiSCs was further empowered by the robust re-activation of Wnt activity. Therefore, during gastrulation, the tuning of the signalling activities that mediate mesendoderm differentiation is instrumental for the acquisition of ectoderm potency in the epiblast.
AB - During embryogenesis, the stringent regulation of Wnt activity is crucial for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore be crucial for the allocation of epiblast cells to ectoderm progenitors during gastrulation. To test this hypothesis, we examined the lineage characteristics of epiblast stem cells (EpiSCs) that were derived and maintained under different signalling conditions. We showed that suppression of Wnt activity enhanced the ectoderm propensity of the EpiSCs. Neuroectoderm differentiation of these EpiSCs was further empowered by the robust re-activation of Wnt activity. Therefore, during gastrulation, the tuning of the signalling activities that mediate mesendoderm differentiation is instrumental for the acquisition of ectoderm potency in the epiblast.
KW - Epiblast stem cells
KW - Head development
KW - Lineage propensity
KW - Neurogenesis
KW - Wnt signalling
U2 - 10.1242/dev.172858
DO - 10.1242/dev.172858
M3 - Journal article
C2 - 30890572
AN - SCOPUS:85064470985
VL - 146
JO - Development
JF - Development
SN - 0950-1991
IS - 7
M1 - dev172858
ER -
ID: 226875938