Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
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- s41467-019-13687-0
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Variations in transcription start site (TSS) selection reflect diversity of preinitiation complexes and can impact on post-transcriptional RNA fates. Most metazoan polymerase II-transcribed genes carry canonical initiation with pyrimidine/purine (YR) dinucleotide, while translation machinery-associated genes carry polypyrimidine initiator (5’-TOP or TCT). By addressing the developmental regulation of TSS selection in zebrafish we uncovered a class of dual-initiation promoters in thousands of genes, including snoRNA host genes. 5’-TOP/TCT initiation is intertwined with canonical initiation and used divergently in hundreds of dual-initiation promoters during maternal to zygotic transition. Dual-initiation in snoRNA host genes selectively generates host and snoRNA with often different spatio-temporal expression. Dual-initiation promoters are pervasive in human and fruit fly, reflecting evolutionary conservation. We propose that dual-initiation on shared promoters represents a composite promoter architecture, which can function both coordinately and divergently to diversify RNAs.
Originalsprog | Engelsk |
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Artikelnummer | 168 |
Tidsskrift | Nature Communications |
Vol/bind | 11 |
Udgave nummer | 1 |
Sider (fra-til) | 1-16 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 2020 |
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