Drosophila Rab2 controls endosome-lysosome fusion and LAMP delivery to late endosomes
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Drosophila Rab2 controls endosome-lysosome fusion and LAMP delivery to late endosomes. / Lund, Viktor Karlovich; Madsen, Kenneth Lindegaard; Kjærulff, Ole.
I: Autophagy, Bind 14, Nr. 9, 2018, s. 1520-1542.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Drosophila Rab2 controls endosome-lysosome fusion and LAMP delivery to late endosomes
AU - Lund, Viktor Karlovich
AU - Madsen, Kenneth Lindegaard
AU - Kjærulff, Ole
PY - 2018
Y1 - 2018
N2 - Rab2 is a conserved Rab GTPase with a well-established role in secretory pathway function and phagocytosis. Here we demonstrate that Drosophila Rab2 is recruited to late endosomal membranes, where it controls the fusion of LAMP-containing biosynthetic carriers and lysosomes to late endosomes. In contrast, the lysosomal GTPase Gie/Arl8 is only required for late endosome-lysosome fusion, but not for the delivery of LAMP to the endocytic pathway. We also find that Rab2 is required for the fusion of autophagosomes to the endolysosomal pathway, but not for the biogenesis of lysosome-related organelles. Surprisingly, Rab2 does not rely on HOPS-mediated vesicular fusion for recruitment to late endosomal membranes. Our work suggests that Drosophila Rab2 is a central regulator of the endolysosomal and macroautophagic/autophagic pathways by controlling the major heterotypic fusion processes at the late endosome.
AB - Rab2 is a conserved Rab GTPase with a well-established role in secretory pathway function and phagocytosis. Here we demonstrate that Drosophila Rab2 is recruited to late endosomal membranes, where it controls the fusion of LAMP-containing biosynthetic carriers and lysosomes to late endosomes. In contrast, the lysosomal GTPase Gie/Arl8 is only required for late endosome-lysosome fusion, but not for the delivery of LAMP to the endocytic pathway. We also find that Rab2 is required for the fusion of autophagosomes to the endolysosomal pathway, but not for the biogenesis of lysosome-related organelles. Surprisingly, Rab2 does not rely on HOPS-mediated vesicular fusion for recruitment to late endosomal membranes. Our work suggests that Drosophila Rab2 is a central regulator of the endolysosomal and macroautophagic/autophagic pathways by controlling the major heterotypic fusion processes at the late endosome.
U2 - 10.1080/15548627.2018.1458170
DO - 10.1080/15548627.2018.1458170
M3 - Journal article
C2 - 29940804
VL - 14
SP - 1520
EP - 1542
JO - Autophagy
JF - Autophagy
SN - 1554-8627
IS - 9
ER -
ID: 201307277